Protein kinase A regulatory subunit is required for normal growth, zoosporogenesis, and pathogenicity in Phytophthora sojae

文献类型： 外文期刊

第一作者： Zhang, Yunxiang

作者： Zhang, Yunxiang;Zhang, Zhuo;Chen, Yue;Tan, Xinqiu;Liu, Yong;Zhang, Xin;Zhang, Deyong;Zhang, Zhuo;Chen, Yue;Tan, Xinqiu;Liu, Yong;Zhang, Xin;Zhang, Deyong;Tian, Zhe;Wang, Jinglin

作者机构：

关键词： Phytophthora sojae; Protein kinase A; Regulatory subunit; Production of zoospores; Pathogenicity

期刊名称：RESEARCH IN MICROBIOLOGY （ 影响因子：3.4； 五年影响因子：3.7 ）

ISSN： 0923-2508

年卷期： 2024 年 175 卷 4 期

页码：

收录情况： SCI

摘要： Phytophthora sojae, one of the most devastating Oomycete pathogens, causes severe diseases that lead to economic loss in the soybean industry. The production of zoospores play a crucial role during the development of Phytophthora disease. In this work, CRISPR/Cas9 genome editing technology were used to obtain protein kinase A regulatory subunit (PsPkaR) knockout mutants. The role of PsPkaR in the production of zoospores and pathogenicity of P. sojae was analyzed. The overall findings indicate that PsPkaR is involved in regulating the growth process of P. sojae, primarily affecting the hyphal morphology and growth rate. Additionally, PsPkaR participates in the regulation of the release process of zoospores. Specifically, knocking-out PsPkaR resulted in incomplete cytoplasmic differentiation and uneven protoplast division, leading to abnormal release of zoospores. Furthermore, when the PsPkaR knockout mutants were inoculated on soybean leaves, the pathogenicity was significantly reduced compared to that of the wild-type and control strains. These findings of this study provide important clues and evidence regarding the role of the cAMP-PKA signaling pathway in the interaction between P. sojae and its host. This work contributes to a better understanding of the pathogenic mechanism of P. sojae and the development of corresponding prevention and control strategies. (c) 2023 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.

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