A hydrogel-based ratiometric fluorescent sensor relying on rhodamine B labelled AIE-featured hyperbranched poly(amido amine) for heparin detection

文献类型： 外文期刊

第一作者： Li, Cheng

作者： Li, Cheng;Zheng, Xiaochun;Chen, Li;Li, Shaobo;Tian, Xiaoxian;Zhang, Dequan;Wang, Yong;Liu, Bai-Tong;Wang, Yi-Ting;Zhang, Tian-Jiao

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关键词： Heparin; Fluorescence; Detection; Hyperbranched poly(amido amine); Hydrogel

期刊名称：ANALYTICA CHIMICA ACTA （ 影响因子：6.2； 五年影响因子：5.9 ）

ISSN： 0003-2670

年卷期： 2024 年 1300 卷

页码：

收录情况： SCI

摘要： The fluorescent flexible sensor for point -of -care quantification of clinical anticoagulant drug, Heparin (Hep), is still an urgent need of breakthrough. In this research, a hyperbranched poly(amido amine) (HPA) was decorated with tetraphenylethene (TPE) and Rhodamine B (RhB), constructing a ratiometric fluorescent sensor (TR-HPA) for Hep. When the sensor was exposed to Hep, the TPE units within the probe skeleton would aggregate, resulting in an increasing fluorescent emission at 483 nm. The 580 nm of fluorescence came from RhB enhance, simultaneously, due to the fluorescence resonance energy transfer. As a result, there are two good linear correlation between the fluorescence emission ratio (E 483 /E 580 ) of TR-HPA and the Hep concentration over a range of 0-1.0 mu M, with a low limit of detection of 3.0 nM. Furthermore, we incorporate the TR-HPA probe into a polyvinyl alcohol (PVA) hydrogel matrix to create a flexible fluorescent sensing system platform, denoted as TRHPA/PVA. This approach offers a straightforward visual detection method by causing a fluorescence color change from pink to blue when trace amounts of Hep are present. The hydrogel-based fluorescent sensor streamlines the detection procedures for Hep in biomedical applications. It shows great potential in rapid and point -of -care human blood clotting condition monitoring, making it suitable for next -generation wearable medical devices.

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