A multiplex and regenerable surface plasmon resonance (MR-SPR) biosensor for DNA detection of genetically modified organisms

文献类型： 外文期刊

第一作者： An, Na

作者： An, Na;Zhang, Yukun;Wen, Tingting;Liu, Weixiao;Li, Liang;Jin, Wujun;Li, Kai;Liu, Gang

作者机构：

关键词： Plasmon resonance biosensor; Genetically modified organisms; Biosensor; Regenerable; Label-free

期刊名称：TALANTA （ 影响因子：6.057； 五年影响因子：5.386 ）

ISSN： 0039-9140

年卷期： 2021 年 231 卷

页码：

收录情况： SCI

摘要： The continuous advancement of analytical technology has provided methods with increasing sensitivity and precision to detect genetically modified organisms (GMOs). Novel analytical strategy-based detection methods are alternatives to conventional polymerase chain reaction (PCR)-mediated assays, which are still the gold standard in this field. However, PCR primers and probes cannot be reused, which makes the technique uneconomical. Surface plasmon resonance (SPR) is an optical and label-free technique for studying ligand-analyte interactions, especially for DNA hybridization, and several SPR biosensors have been described for the detection of nucleic acids. Here, a multiplexed, regenerable and real-time SPR biosensor for the detection of GMOs is described. A biosensor was constructed for qualitative detection of T-nos, CaMV35S and cry1A and had good specificity and sensitivity. The limit of detection (LOD) of this biosensor was 0.1 nM without any signal amplification. Furthermore, our biosensor could be stably regenerated more than 100 times over at least 20 days and showed good reproducibility. This nucleic acid SPR biosensor has potential for application in other types of biological detection.

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