Data-dependent acquisition based high-resolution mass spectrum for trace Alternaria mycotoxin analysis and sulfated metabolites identification

文献类型： 外文期刊

第一作者： Zhang, Xiaoman

作者： Zhang, Xiaoman;Qu, Jinyao;Lin, Yucheng;Lu, Guozhu;You, Yanli;Li, Yanshen;Dai, Zhaoji;Dai, Zhaoji;Yang, Shupeng;Liu, Huihui;Wu, Yongning;Jiang, Guibin

作者机构：

关键词： Data-dependent acquisition; Alternaria mycotoxin; Sulfated metabolites; High-resolution mass spectrometer; Food safety

期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）

ISSN： 0308-8146

年卷期： 2021 年 364 卷

页码：

收录情况： SCI

摘要： Alternaria mycotoxins are food-related compounds that are mainly produced by Alternaria fungi species. However, it's difficult for Alternaria mycotoxins analysis, especially for conjugated metabolites in food safety surveillance. In this work, a novel data-dependent acquisition (DDA) full mass scan and products scan protocol was proposed for qualitative and quantitative analysis of five target mycotoxins in tomato samples using ultrahigh-performance liquid chromatography coupled with quadrupole Orbitrap high-resolution mass spectrometry (UHPLC-Q-Orbitrap). In total, 24 sulfated metabolites were detected with post-data analysis techniques, and two sulfated metabolites (AME-sulfated and AOH-sulfated) were identified in Alternaria fungi -inoculated tomatoes. In addition, a custom database was established, and it was successfully applied for Alternaria mycotoxins and sulfated metabolites screening in tomatoes. With the improvement in high-resolution mass spectrometry (HRMS) as well as post-data analysis techniques, DDA based HRMS method could be widely applied for compound analysis, identification, and screening in quantitative field.

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