Production of viable chicken by allogeneic transplantation of primordial germ cells induced from somatic cells

文献类型： 外文期刊

第一作者： Zhao, Ruifeng

作者： Zhao, Ruifeng;Zuo, Qisheng;Yuan, Xia;Jin, Kai;Jin, Jing;Ding, Ying;Zhang, Chen;Li, Tingting;Jiang, Jingyi;Li, Jiancheng;Zhang, Ming;Shi, Xiang;Sun, Hongyan;Zhang, Yani;Xu, Qi;Chang, Guobin;Wu, Xinsheng;Zhang, Yang;Chen, Guohong;Li, Bichun;Zhao, Ruifeng;Zuo, Qisheng;Yuan, Xia;Jin, Kai;Jin, Jing;Ding, Ying;Zhang, Chen;Li, Tingting;Jiang, Jingyi;Li, Jiancheng;Zhang, Ming;Shi, Xiang;Sun, Hongyan;Zhang, Yani;Xu, Qi;Chang, Guobin;Wu, Xinsheng;Zhang, Yang;Chen, Guohong;Li, Bichun;Zhao, Zhenhua;Li, Bing;Song, Jiuzhou

作者机构：

期刊名称：NATURE COMMUNICATIONS （ 影响因子：14.919； 五年影响因子：15.805 ）

ISSN： 2041-1723

年卷期： 2021 年 12 卷 1 期

页码：

收录情况： SCI

摘要： The allogeneic transplantation of primordial germ cells (PGCs) derived from somatic cells overcomes the limitation of avian cloning. Here, we transdifferentiate chicken embryo fibroblasts (CEFs) from black feathered Langshan chickens to PGCs and transplant them into White Plymouth Rock chicken embryos to produce viable offspring with characteristics inherited from the donor. We express Oct4/Sox2/Nanog/Lin28A (OSNL) to reprogram CEFs to induced pluripotent stem cells (iPSCs), which are further induced to differentiate into PGCs by BMP4/BMP8b/EGF. DNA demethylation, histone acetylation and glycolytic activation elevate the iPSC induction efficiency, while histone acetylation and glycolytic inhibition facilitate PGCs formation. The induced PGCs (iPGCs) are transplanted into the recipients, which are self-crossed to produce 189/509 somatic cells derived chicken with the donor's characteristics. Microsatellite analysis and genome sequencing confirm the inheritance of genetic information from the donor. Thus, we demonstrate the feasibility of avian cloning from somatic cells.

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