Enhanced Bio-Barcode Immunoassay Using Droplet Digital PCR for Multiplex Detection of Organophosphate Pesticides

文献类型： 外文期刊

第一作者： Cui, Xueyan

作者： Cui, Xueyan;Zhang, Chan;Xu, Lingyuan;Wang, Yuanshang;Cao, Zhen;She, Yongxin;Jin, Fen;Wang, Jing;Jin, Maojun;Abd El-Aty, A. M.;Abd El-Aty, A. M.;Abd El-Aty, A. M.;Liu, Haijin;Jia, Huiyan;Salvador, J-Pablo;Salvador, J-Pablo;Hammock, Bruce D.;Hammock, Bruce D.

作者机构：

关键词： gold nanoparticles; magnetic nanoparticles; oligonucleotides; ddPCR; organophosphate pesticides

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

ISSN： 0021-8561

年卷期： 2021 年 69 卷 37 期

页码：

收录情况： SCI

摘要： A bio-barcode immunoassay based on droplet digital polymerase chain reaction (ddPCR) was developed to simultaneously quantify triazophos, parathion, and chlorpyrifos in apple, cucumber, cabbage, and pear. Three gold nanoparticle (AuNP) probes and magnetic nanoparticle (MNP) probes were prepared, binding through their antibodies with the three pesticides in the same tube. Three groups of primers, probes, templates, and three antibodies were designed to ensure the specificity of the method. Under the optimal conditions, the detection limits (expressed as IC10) of triazophos, parathion, and chlorpyrifos were 0.22, 0.45, and 4.49 ng mL(-1), respectively. The linear ranges were 0.01-20, 0.1-100, and 0.1-500 ng mL(-1), and the correlation coefficients (R-2) were 0.9661, 0.9834, and 0.9612, respectively. The recoveries and relative standard deviations (RSDs) were in the ranges of 75.5-98.9 and 8.3-16.7%. This study provides the first insights into the ddPCR for the determination of organophosphate pesticides. It also laid the foundation for high-throughput detection of other small molecules.

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