Lipid homeostasis is essential for oogenesis and embryogenesis in the silkworm, Bombyx mori

文献类型： 外文期刊

第一作者： Yang, Fangying

作者： Yang, Fangying;Hu, Bo;Zhang, Zhongjie;Chen, Kai;Yu, Ye;Tan, Anjiang;Hu, Bo;Zhang, Zhongjie;Chen, Kai;Yu, Ye;Tan, Anjiang;Xu, Xiaoyan;Bai, Hua

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关键词： Bombyx mori; Lipid metabolism; Lipidomics; Fecundity

期刊名称：CELLULAR AND MOLECULAR LIFE SCIENCES （ 影响因子：8.0； 五年影响因子：8.7 ）

ISSN： 1420-682X

年卷期： 2024 年 81 卷 1 期

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收录情况： SCI

摘要： Reproduction, a fundamental feature of all known life, closely correlates with energy homeostasis. The control of synthesizing and mobilizing lipids are dynamic and well-organized processes to distribute lipid resources across tissues or generations. However, how lipid homeostasis is precisely coordinated during insect reproductive development is poorly understood. Here we describe the relations between energy metabolism and reproduction in the silkworm, Bombyx mori, a lepidopteran model insect, by using CRISPR/Cas9-mediated mutation analysis and comprehensively functional investigation on two major lipid lipases of Brummer (BmBmm) and hormone-sensitive lipase (BmHsl), and the sterol regulatory element binding protein (BmSrebp). BmBmm is a crucial regulator of lipolysis to maintain female fecundity by regulating the triglyceride (TG) storage among the midgut, the fat body, and the ovary. Lipidomics analysis reveals that defective lipolysis of females influences the composition of TG and other membrane lipids in the BmBmm mutant embryos. In contrast, BmHsl mediates embryonic development by controlling sterol metabolism rather than TG metabolism. Transcriptome analysis unveils that BmBmm deficiency significantly improves the expression of lipid synthesis-related genes including BmSrebp in the fat body. Subsequently, we identify BmSrebp as a key regulator of lipid accumulation in oocytes, which promotes oogenesis and cooperates with BmBmm to support the metabolic requirements of oocyte production. In summary, lipid homeostasis plays a vital role in supporting female reproductive success in silkworms.

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