The effective expression of xylanase gene in Candida utilis by 18S rDNA targeted homologous recombination in pGLR9K

文献类型： 外文期刊

第一作者： Wang Wei

作者： Wang Wei;Yang Hong-Lan;Bao HuiFang;Shan Qi-mu-ge;Yang Hong-Lan;Zhang Daoyuan;Wang Wei;Shan Qi-mu-ge;Wood, Andrew J.

作者机构：

关键词： recombinant DNA;Candida utilis.;Expression of xylanase;18S rDNA;Xylanase activity;Bioreactor;Internet resource.

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

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收录情况： SCI

摘要： In order to test whether 18S rDNA can influence positively xylanase gene effective expression in the yeast of Candida utilis, a targeting vector pGLR9K-XA was constructed by adding an interested gene xynA from Streptomyces olivaceoviridis into the vector pGLR9K which is constructed by ourselves. pGLR9K contains the 18S rDNA, GAP promoter and CYH resistance gene sequence, all of which is from C. utilis. Then the vector pGLR9K-XA was transformed into C. utilis. To test the vector and transformed system, PCR, Southern blot and DNS methods were used. The results showed that xylanase gene can be detected in the chromosome DNA of recombinant C. utilis and the enzyme activity of xylanase is up to 60 IU mlp# in the study. It is suggested that this system can be used to express exogenous genes in C. utilis as a bioreactors. This is the first report that xylanase gene was expressed in C. utilis.

分类号： Q7

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