GENERATION AND CHARACTERIZATION OF 11 NOVEL EST DERIVED MICROSATELLITES FROM VICIA FABA (FABACEAE)

文献类型: 外文期刊

第一作者: Gong, Ya-Ming

作者: Gong, Ya-Ming;Xu, Sheng-Chun;Hu, Qi-Zan;Zhang, Gu-Wen;Ding, Ju;Mao, Wei-Hua;Li, Ze-Yun

作者机构:

关键词: heterozygosity;genetic diversity;NCBI database;resource conservation

期刊名称:AMERICAN JOURNAL OF BOTANY ( 影响因子:3.844; 五年影响因子:3.657 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Premise of the study: Simple sequence repeat (SSR) markers were developed for faba bean using expressed sequence tags (ESTs) from the NCBI database to study for genetic diversity.Methods and Results: A total of 11 novel EST-SSR loci were generated and characterized when tested on four populations of 29 faba bean individuals from China and Europe. The number of alleles (A) ranged from 1 to 3 in each population, and observed heterozygosity (H-O) and expected heterozygosity (H-E) ranged from 0 to 0.5000 and 0.6400, respectively. Furthermore, transferable analysis revealed that eight of these loci (72.73%) amplified in Pisum sativum L., six of which (75.00%) detected polymorphism.Conclusions: The developed markers in this study will provide valuable tools for genetic diversity, resource conservation, genetic mapping, and marker-assisted breeding of faba bean in the future.

分类号: Q94

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