Validation of reference genes of grass carp Ctenopharyngodon idellus for the normalization of quantitative real-time PCR
文献类型: 外文期刊
第一作者: Ye, Xing
作者: Ye, Xing;Zhang, Lili;Tian, Yuanyuan;Lao, Haihua;Bai, Junjie;Yu, Lingyun;Dong, Haiyan;Dong, Haiyan
作者机构:
关键词: gene expression;Ctenopharyngodon idella.;Bacterial infection;Quantitative real-time PCR;Reference gene;Internet resource.
期刊名称:BIOTECHNOLOGY LETTERS ( 影响因子:2.461; 五年影响因子:2.457 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Expression of four reference genes of grass carp, including o-actin (ACTB), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), 18S rRNA (18S) and elongation factor-1 alpha (EF1l), was studied in tissues of normal individuals and bacteria-infected individuals. EF1l had the most stable expressions followed by 18S rRNA then GAPDH; ACTB had the least stability. After being infected with bacteria, the grass carp showed minimal changes in expression levels of EF1l in the liver and head kidney, while ACTB had the most stable expressions in spleen but the least stable in liver. EF1l is thus the optimal reference gene in quantitative real-time PCR analysis to quantitate the expression levels of target genes in tissues of grass carp.
分类号: Q7
- 相关文献
作者其他论文 更多>>
-
Full-Length Transcriptome Sequencing and Comparative Transcriptomics Reveal the Molecular Mechanisms Underlying Gonadal Development in Sleepy Cod (Oxyeleotris lineolata)
作者:Fan, Jiajia;Ma, Dongmei;Zhu, Huaping;Lin, Minghui;Zhong, Zaixuan;Tian, Yuanyuan;Fan, Jiajia;Ma, Dongmei;Zhu, Huaping;Lin, Minghui;Zhong, Zaixuan;Tian, Yuanyuan
关键词:sleepy cod Oxyeleotris lineolata; sexual dimorphism; transcriptome; sex determination; gonadal differentiation
-
Investigating the influence of the molecular structure and physiochemical properties of starches from glutinous and japonica sorghum on light-flavor liquor fermentation
作者:Ma, Zhenbing;Wang, Chenyang;Tian, Yuanyuan;Bian, Minghong;Ma, Yi;Lan, Haibo;Ma, Zhenbing;Wang, Chenyang;Tian, Yuanyuan;Bian, Minghong;Ma, Yi;Lan, Haibo;Zhao, Dong;Zheng, Jia;Su, Jian;Wang, Jiaxu;Ke, Fulai
关键词:Sorghum starch; Fine structure; Pasting properties; Gelatinization properties; Thermal properties
-
A TMT-Based Proteomic Analysis of Osmoregulation in the Gills of Oreochromis mossambicus Exposed to Three Osmotic Stresses
作者:Su, Huanhuan;Ma, Dongmei;Fan, Jiajia;Zhong, Zaixuan;Tian, Yuanyuan;Zhu, Huaping;Su, Huanhuan;Ma, Dongmei;Fan, Jiajia;Zhong, Zaixuan;Tian, Yuanyuan;Zhu, Huaping
关键词:TMT-based proteomics; gills; osmotic stresses;
Oreochromis mossambicus -
Evolution and transmission potential of iuc3-positive virulence plasmids in hypervirulent Klebsiella pneumoniae
作者:Wang, Jiayun;Wang, Heng;Wang, Jiayun;Ji, Xing;Li, Jun;Zhang, Lili;Wang, Ran;He, Tao;Garcia, Pilar
关键词:Klebsiella pneumoniae; HvKP; iucABCD; Genomic evolution; Conjugation; Plasmid fusion
-
Effects of different feeding strategies for the frozen Antarctic krill meal on growth, muscle nutrition, digestive enzyme activity, immunity, molting, and gut microbiota structure of Cherax quadricarinatus
作者:Mai, Zhuang;Liu, Fubai;Yang, Tianyan;Mai, Zhuang;Wei, Jie;Wang, Yakun;Liu, Fubai;Li, Huarong;Su, Qiyao;Hong, Kunhao;Zhou, Qiaoyan;Jiao, Tianhui;Yu, Lingyun;Zeng, Zhongqiang;Li, Huarong;Su, Qiyao;Hong, Kunhao;Jiao, Tianhui
关键词:Antarctic krill meal; Cherax quadracarinatus; growth performance; fatty acid; immunity-related gene
-
Role of myostatin promoter mutations in growth and molting regulation of Macrobrachium rosenbergii
作者:Su, Qiyao;Wei, Jie;Wang, Yakun;Liufu, Bai;Li, Huarong;Mai, Zhuang;Hong, Kunhao;Zhou, Qiaoyan;Jiao, Tianhui;Mu, Xidong;Yu, Lingyun;Su, Qiyao;Li, Huarong;Hong, Kunhao;Jiao, Tianhui;Liufu, Bai;Mai, Zhuang
关键词:
Macrobrachium rosenbergii ;myostatin ; RNAi; molt; growth -
Integrated Metabolomic and Transcriptomic Analyses Reveal the Potential Molecular Mechanism Underlying Callus Browning in Paeonia ostii
作者:Wen, Xiaohui;Xu, Wenting;Zhang, Lili;Shi, Xiaohua;Zhou, Jianghua;Liu, Huichun;Zhu, Kaiyuan
关键词:callus browning;
P. ostii ; phenol compounds; flavonoid biosynthesis; cellular totipotency regulators