Expression, purification and monoclonal antibodies preparation of recombinant equine mature interleukin-18

文献类型： 外文期刊

第一作者： Tong, Tiegang

作者： Tong, Tiegang;Bai, Yu;Liu, Guangliang;Wang, Qun;Zhang, Weijun;Xiao, Yihong;Xu, Shulan;Liu, Nihong;Yang, Tao;Wu, Donglai

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关键词： interleukin-18;monoclonal antibodies: expression;pupurification;Gene Name;human IFN-gamma gene [Hominidae]: human interferon-gamma gene;human rhIL-12 gene [Hominidae]: expression

期刊名称：VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY （ 影响因子：2.046； 五年影响因子：2.217 ）

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收录情况： SCI

摘要： IL-18 is a cytokine originally discovered as an important modulator of immune responses and subsequently shown to be pleiotropic. In this report, we expressed the recombinant equine mature interleukin-18 (rEMIL-18) in E. coli and purified it by nickel affinity gel column chromatography. Purified rEMIL-18 had biological activity commensurate with recombinant human IL-18, as determined by its synergistic effect with recombinant human IL-12 (rhIL-12) on the induction of IFN-gamma gene expression in equine peripheral blood mononuclear cells (PBMC). Following intraperitoneal (i.p.) immunization of BALB/c mice with rEMIL-18, nine monoclonal antibodies (mAbs) against equine interleukin-18 (EIL-18) were obtained and characterized. These mAbs recognized different epitopes on equine mature interleukin-18 (EMIL-18) protein based on their reactivity with two peptides containing different amino acid sequences and one of these mAbs has neutralization activity against EIL-18 in an IFN-gamma-induction assay

分类号： S85

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