Padi6 expression patterns in buffalo oocytes and preimplantation embryos

文献类型： 外文期刊

第一作者： Sun, Qinqiang

作者： Sun, Qinqiang;Yang, Yuan;Zhang, Yue;Chen, Dongrong;Fu, Qiang;Zheng, Haiying;Qin, Guangsheng

作者机构：

关键词： maternal -effect genes; Padi6; oocyte; preimplantation embryo; immunofluorescence.

期刊名称：ANIMAL REPRODUCTION （ 影响因子：1.7； 五年影响因子：1.9 ）

ISSN： 1806-9614

年卷期： 2024 年 21 卷 1 期

页码：

收录情况： SCI

摘要： The subcortical maternal complex, which consists of maternal-effect genes, plays a crucial role in the development of oocytes and preimplantation embryo until the activation of the zygote genome. One such gene, known as peptidyl-arginine deiminase VI (Padi6), is involved in the oocyte maturation, fertilization and embryonic development. However, the precise function of Padi6 gene in buffalo is still unclear and requires further investigation. In this study, the sequence, mRNA and protein expression patterns of Padi6 gene were analyzed in oocytes, preimplantation embryos and somatic tissues of buffalo. The coding sequence of gene was successfully cloned and characterized. Real-time quantitative PCR results indicated an absence of Padi6 transcripts in somatic tissues. Notably, the expression levels of Padi6 in oocytes showed an increased from the germinal vesicle stage to metaphase II stage, followed by a rapid decrease during the morula and blastocyst stages. Immunofluorescence analysis confirmed these findings, revealing a noticeable decline in protein expression levels. Our research provides the initial comprehensive expression profile of Padi6 in buffalo oocytes and preimplantation embryos, serving as a solid foundation for further investigations into the functionality of maternal-effect genes in buffalo.

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