SlDREB2 gene specifically recognizing to the universal DRE elements is a transcriptional activator improving drought tolerance in tomato

文献类型： 外文期刊

第一作者： Tao, Leyuan

作者： Tao, Leyuan;Chen, Hongna;Wang, Bing;Jiang, Linlin;Cheng, Xian-Guo;Yu, Guohong;Chen, Hongna;Han, Xiaori;Lin, Guolin

作者机构：

关键词： Tomato; Dre cis-elements; Drought stress; SlDREB2transcription factor; Gene silencing

期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：4.342； 五年影响因子：4.342 ）

ISSN： 0304-4238

年卷期： 2022 年 295 卷

页码：

收录情况： SCI

摘要： Plant DREB transcription factors play an important transcriptional activation role under abiotic stresses. In this study, both tomato RNAi-lines and transgenic Arabidopsis lines were evaluated to unveil whether the SlDREB2 gene functions by the transcriptional activation pathway under drought stress. The SlDREB2 gene contains a typical AP2/EREBP conserved domain and is localized to the cell nucleus. Data shows that the SlDREB2 gene almost revealed identical expression profile in all tissues of tomato seedlings and was induced by drought. In vitro assay reveals that the SlDREB2 protein specifically bound to the universal DRE cis-elements, instead of limiting to the DRE motifs in the promoters of the downstream genes. The transcripts of the DREB2 gene in RNAi-lines were lowered by more than 55%, and proline in RNAi-lines were lowered by more than 25%, while soluble sugar was decreased by more than 40% in RNAi-lines comparing to the WT after two weeks of drought stress. However, the MDA contents in RNAi-lines at least demonstrated an increase of 0.8 folds after three weeks of drought stress comparing to the WT. The contents of proline in Arabidopsis overexpressing the SlDREB2 gene increased 12.4% in OE-1 line and 24.8% in OE-2 line comparing to the WT after 2 weeks of drought stress, respectively. Over-expression of the SlDREB2 gene up-regulated 1.98-fold of the Kin1 transcripts in OE-1 line and 2.53-fold of the Kin2 gene transcripts in OE-2 line, while the rd29A gene was up-regulated by 2.91-fold in OE-1 line and 9.63-fold in OE-2 line comparing to the WT after two weeks of drought stress, respectively. Specific up-regulation of the SlDREB2 gene in Arabidopsis enriched the functional genes involving in the metabolic process under drought stress. This study provided novel insight into the transcription activation pathway of the SlDREB2 gene in improving plant drought stress tolerance.

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[1]SlDREB2 gene specifically recognizing to the universal DRE elements is a transcriptional activator improving drought tolerance in tomato. Tao, Leyuan,Chen, Hongna,Wang, Bing,Jiang, Linlin,Cheng, Xian-Guo,Yu, Guohong,Chen, Hongna,Han, Xiaori,Lin, Guolin. 2022

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