Transcriptome Profiling to Identify Genes Involved in Non-Target-Site-Based Resistance to Clodinafop-Propargyl in Asia Minor Bluegrass (Polypogon fugax)

文献类型： 外文期刊

第一作者： Zhou, Fengyan

作者： Zhou, Fengyan;Meng, Ziwei;Han, Yunjing;Zhang, Yong;Meng, Ziwei;Tang, Wei

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关键词： Polypogon fugax; ACCase herbicide resistant; non-target-site-based resistance (NTSR); transcriptome analysis; seedling stage; tillering stage

期刊名称：AGRONOMY-BASEL （ 影响因子：3.949； 五年影响因子：4.117 ）

ISSN：

年卷期： 2022 年 12 卷 12 期

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收录情况： SCI

摘要： Asia Minor bluegrass (Polypogon fugax Nees ex Steud.) is a problematic grass weed of winter crops in China, where some populations have become resistant to herbicides. Previously, we identified a P. fugax population QS exhibiting target-site-based resistance (TSR) and non-target-site-based resistance (NTSR) to clodinafop-propargyl. This study aims to understand the metabolic resistance to clodinafop-propargyl between susceptible (XC) and resistant (QS) populations of P. fugax in the seedling and tillering stage, separately. Several differentially expressed candidate genes in the seedling and tillering stages were identified by RNA-Seq, including three P450 family genes, one glutathione S-transferase (GST) gene, and two ATP-binding cassette transporters. Additionally, we discovered a GST gene that was significantly differentially expressed in the resistant population during the seedling stage, as well as three peroxidase genes that were presumed to be related to NTSR metabolism. Three other peroxidase genes and one esterase were presumed to be related to NTSR metabolism during the tillering stage of the resistant population. Overexpression of the three randomly selected candidate genes can enhance herbicide-resistance in Arabidopsis transgenic plants. This study provided a novel insight into herbicide metabolism regulation genes during the different growth stages of resistant P. fugax population.

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