Identification of Candidate Genes Associated with Type-II Sex Pheromone Biosynthesis in the Tea Geometrid (Ectropis obliqua) (Lepidoptera: Geometridae)

文献类型： 外文期刊

第一作者： Xu, Changxia

作者： Xu, Changxia;Fu, Nanxia;Cai, Xiaoming;Li, Zhaoqun;Bian, Lei;Xiu, Chunli;Chen, Zongmao;Luo, Zongxiu;Xu, Changxia;Fu, Nanxia;Cai, Xiaoming;Li, Zhaoqun;Bian, Lei;Xiu, Chunli;Chen, Zongmao;Luo, Zongxiu;Xu, Changxia;Ma, Long

作者机构：

关键词： transcriptomic analyses; gene identification; sex pheromone; biosynthesis; RT-qPCR; Ectropis obliqua

期刊名称：INSECTS （ 影响因子：3.0； 五年影响因子：3.1 ）

ISSN：

年卷期： 2024 年 15 卷 4 期

页码：

收录情况： SCI

摘要： The tea geometrid (Ectropis obliqua (Prout)) is a major defoliator of tea plants and seriously affects the tea quality and yield in China. The females release three Type-II sex pheromone components for mate communication, but knowledge about the biosynthesis of these compounds is still limited. In this study, we screened for the candidate genes in the sex pheromone biosynthetic pathway with a combination of comparative RNAseq, phylogenetic relationship, and tissue expression pattern analyses. A total of seven tentative biosynthetic enzymes were identified, including two ELOs (ELO3 and ELO5), two FARs (FAR2 and FAR9), one DEC (CYP4G173), one LIP (LIP1), and one epoxidase (CYP340BD1). Overall, our results provide a foundation for further functional elucidation of the vital genes involved in E. obliqua sex pheromone biosynthesis. Ectropis obliqua, a notorious tea pest, produces a Type-II sex pheromone blend for mate communication. This blend contains (Z,Z,Z)-3,6,9-octadecatriene, (Z,Z)-3,9-cis-6,7-epoxy-octadecadiene, and (Z,Z)-3,9-cis-6,7-epoxy-nonadecadiene. To elucidate the genes related to the biosynthesis of these sex pheromone components, transcriptome sequencing of the female E. obliqua pheromone gland and the abdomen without pheromone gland was performed. Comparative RNAseq analyses identified 52 putative genes, including 7 fatty acyl-CoA elongases (ELOs), 9 fatty acyl-CoA reductases (FARs), 1 decarbonylase (DEC), 3 lipophorins (LIPs), and 32 cytochrome P450 enzymes (CYPs). Tissue expression profiles revealed that two ELOs (ELO3 and ELO5), two FARs (FAR2 and FAR9), one DEC (CYP4G173), and one LIP (LIP1) displayed either abdomen-centric or -specific expression, suggesting potential roles in sex pheromone biosynthesis within the oenocytes of E. obliqua. Furthermore, the tissue expression patterns, combined with phylogenetic analysis, showed that CYP340BD1, which was expressed specifically and predominantly only in the pheromone gland, was clustered with the previously reported epoxidases, highlighting its potential role in the epoxidation of the unsaturated polytriene sex pheromone components. Collectively, our research provides valuable insights into the genes linked to sex pheromone biosynthesis.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序