Construction and analysis of Sip1Aa insecticidal protein random recombination library

文献类型： 外文期刊

第一作者： Ding, Yue-ming

作者： Ding, Yue-ming;Wang, Jing;Wang, Lin;Gao, Ji-Guo;Liu, Rong-Mei;Li, Hai-Tao;Li, Hai-Tao

作者机构：

关键词： Bacillus thuringiensis; Sip1Aa; Random recombination library; Directed evolution; Colaphellus bowringi Baly

期刊名称：JOURNAL OF ASIA-PACIFIC ENTOMOLOGY （ 影响因子：1.303； 五年影响因子：1.427 ）

ISSN： 1226-8615

年卷期： 2021 年 24 卷 2 期

页码：

收录情况： SCI

摘要： The Sip1Aa protein from Bacillus thuringiensis is highly toxic to Colaphellus bowringi Baly. In order to obtain mutant proteins with higher insecticidal activity, a random recombinant library of Sip1Aa protein was constructed using error-prone PCR. A total number of 100 positive transformants were randomly selected for sequence determination, and 25 mutants (M1 to M25) were selected and expressed the respective Sip1Aa mutants. These Sip1Aa variants had a total of 29 base mutations, with an average of 1.2 base mutations per mutant. Compared with that of the wild-type Sip1Aa protein, the insecticidal activity of the mutants M1 (A31G, Y118C, D227E), M5 (K168R) and M21 (I307T) was significantly decreased, with and LC50 values 4 to 6 times higher than the Sip1Aa protein. The mutant M8 (R174S) showed increase in the insecticidal activity against the Colaphellus bowringi Baly was obtained, with an LC50 value 4-fold less than the Sip1Aa protein. The results of this study provide reference for the molecular modification of Sip1Aa protein and the study of key sites of its insecticidal activity.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序