Intestine microbiota and SCFAs response in naturally Cryptosporidium-infected plateau yaks

文献类型： 外文期刊

第一作者： Dong, Hailong

作者： Dong, Hailong;Wu, Qingxia;Chen, Xiushuang;Zhao, Xiaoxiao;Zhao, Chenxi;Li, Kun;Chen, Xiushuang;Zhao, Xiaoxiao;Zhao, Chenxi;Li, Kun;Mehmood, Khalid;Kulyar, Muhammad Fakhar-e-Alam;Bhutta, Zeeshan Ahmad;Zeng, Jiangyong;Nawaz, Shah

作者机构： Tibet Agr & Anim Husb Coll, Key Lab Clin Vet Med Tibet, Linzhi, Xizang, Peoples R China;Nanjing Agr Univ, Inst Tradit Chinese Vet Med, Coll Vet Med, Nanjing, Peoples R China;Nanjing Agr Univ, Coll Vet Med, MOE Joint Int Res Lab Anim Hlth & Food Safety, Nanjing, Peoples R China;Islamia Univ Bahawalpur, Fac Vet & Anim Sci, Dept Clin Med & Surg, Bahawalpur, Pakistan;Huazhong Agr Univ, Coll Vet Med, Wuhan, Peoples R China;Chungbuk Natl Univ, Coll Vet Med, Lab Biochem & Immunol, Cheongju, Chungbuk, South Korea;Tibet Acad Agr & Anim Husb Sci, Inst Anim Husb & Vet Med, Lhasa, Peoples R China;Univ Agr Faisalabad, Fac Vet Sci, Dept Anat, Faisalabad, Pakistan

关键词： Cryptosporidium; yaks; diarrhea; microbiota; SCFAs

期刊名称：FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY （ 2022影响因子：5.7； 五年影响因子：5.9 ）

ISSN： 2235-2988

年卷期： 2023 年 13 卷

页码：

收录情况： SCI

摘要： Diarrhea is a severe bovine disease, globally prevalent in farm animals with a decrease in milk production and a low fertility rate. Cryptosporidium spp. are important zoonotic agents of bovine diarrhea. However, little is known about microbiota and short-chain fatty acids (SCFAs) changes in yaks infected with Cryptosporidium spp. Therefore, we performed 16S rRNA sequencing and detected the concentrations of SCFAs in Cryptosporidium-infected yaks. Results showed that over 80,000 raw and 70,000 filtered sequences were prevalent in yak samples. Shannon (p<0.01) and Simpson (p<0.01) were both significantly higher in Cryptosporidium-infected yaks. A total of 1072 amplicon sequence variants were shared in healthy and infected yaks. There were 11 phyla and 58 genera that differ significantly between the two yak groups. A total of 235 enzymes with a significant difference in abundance (p<0.001) were found between healthy and infected yaks. KEGG L3 analysis discovered that the abundance of 43 pathways was significantly higher, while 49 pathways were significantly lower in Cryptosporidium-infected yaks. The concentration of acetic acid (p<0.05), propionic acid (p<0.05), isobutyric acid (p<0.05), butyric acid (p<0.05), and isovaleric acid was noticeably lower in infected yaks, respectively. The findings of the study revealed that Cryptosporidium infection causes gut dysbiosis and results in a significant drop in the SCFAs concentrations in yaks with severe diarrhea, which may give new insights regarding the prevention and treatment of diarrhea in livestock.

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