Cloning, Expression, and Characterization of Protease-resistant Xylanase from Streptomyces fradiae var. k11
文献类型: 外文期刊
第一作者: Ning, Li
作者: Ning, Li;Yang, Peilong;Wang, Yaru;Luo, Huiying;Meng, Kun;Yao, Bin;Wu, Ningfeng;Fan, Yunliu
作者机构:
关键词: Xylanase;Streptomyces fradiae;protease resistance
期刊名称:JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY ( 影响因子:2.351; 五年影响因子:2.65 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: The gene SfXyn10, which encodes a proteaseresistant xylanase, was isolated using colony PCR screening from a genomic library of a feather-degrading bacterial strain Streptomyces fradiae var. k11. The full-length gene consists of 1,437 bp and encodes 479 amino acids, which includes 41 residues of a putative signal peptide at its N terminus. The amino acid sequence shares the highest similarity (80%) to the endo-1,4-モ-xylanase from Streptomyces coelicolor A3, which belongs to the glycoside hydrolase family 10. The gene fragment encoding the mature xylanase was expressed in Escherichia coli BL21 (DE3). The recombinant protein was purified to homogeneity by acetone precipitation and anionexchange chromatography, and subsequently characterized. The optimal pH and temperature for the purified recombinant enzyme were 7.8 and 60oC, respectively. The enzyme showed stability over a pH range of 4-10. The kinetic values on oat spelt xylan and birchwood xylan substrates were also determined. The enzyme activity was enhanced by Fe2+ and strongly inhibited by Hg2+ and SDS. The enzyme also showed resistance to neutral and alkaline proteases. Therefore, these characteristics suggest that SfXyn10 could be an important candidate for protease-resistant mechanistic research and has potential applications in the food industry, cotton scouring, and improving animal nutrition.
分类号: Q93
- 相关文献
作者其他论文 更多>>
-
Fluorescent Probes Based on Ag NPs@N/GQDs and Molecularly Imprinted Polymer for Sensitive Detection of Noradrenaline in Bananas
作者:Wang, Yaru;Wang, Mingyue;Liu, Sixin;Wang, Yaru;Li, Shuhuai;Ma, Xionghui;Pang, Chaohai;Wu, Yuwei;Wang, Mingyue;Wang, Yaru;Li, Shuhuai;Wang, Mingyue;Li, Bei
关键词:Fluorescent probe; Graphene quantum dots; Silver nanoparticles; Molecular imprinted polymer; Noradrenaline
-
High-Throughput Screening Techniques for the Selection of Thermostable Enzymes
作者:Li, Lanxue;Bai, Yingguo;Yao, Bin;Luo, Huiying;Tu, Tao;Liu, Xiaoqing
关键词:high-throughput screening; virtual screening; protein engineering; enzyme thermostability
-
Recombinant production of SAG1 fused with xylanase in Pichia pastoris induced higher protective immunity against Eimeria tenella infection in chicken
作者:Liu, Chen;Wei, HanBing;Wang, Yuan;Su, Xiaoyun;Tu, Tao;Luo, Huiying;Yao, Bin;Huang, Huoqing;Zhang, Honglian;Liang, Ruiying;Ding, Jiabo;Tang, Xinming
关键词:
-
Cooperative Fermentation Using Multiple Microorganisms and Enzymes Potentially Enhances the Nutritional Value of Spent Mushroom Substrate
作者:Zhang, Anrong;He, Weizhao;Han, Yunsheng;Zheng, Aijuan;Chen, Zhimin;Meng, Kun;Yang, Peilong;Liu, Guohua
关键词:fermentation; Pleurotus eryngii; nutritional value; condition optimization; enzymatic treatment
-
Comparative assessment of morphological, cytological, and reproductive characteristics of the spontaneous mixoploid 'MM 2 x /4 x' and its diploid counterpart 'Miss Manilla ' in Bougainvillea ( Bougainvillea x buttiana Honlttum & Standl.)
作者:Li, Haiyan;Leng, Qingyun;Sun, Luping;Lu, Jinping;Xu, Shisong;Wang, Yaru;Liu, Shaoshan;Yin, Junmei;Niu, Junhai;Li, Haiyan;Wang, Yaru;Yin, Junmei;Niu, Junhai;Leng, Qingyun;Wang, Yaru;Niu, Junhai;Yin, Junmei;Lopez, Hernan Ariel;Lopez, Hernan Ariel;Ma, Chao
关键词:Bougainvillea; Mixoploid; Morphological traits; Cytological analysis; Hybridization; Polyploid breeding
-
Enhancing the endo-activity of the thermophilic chitinase to yield chitooligosaccharides with high degrees of polymerization
作者:Zhang, Ruohan;Zhang, Honglian;Tu, Tao;Luo, Huiying;Yao, Bin;Tian, Jian;Huang, Huoqing;Guan, Feifei;Tian, Xiaoqian;Zhang, Yan;Wu, Ningfeng;Tian, Jian;Tian, Xiaoqian;Zhang, Yan;Sun, Jilu
关键词:Chitin; Chitinase; Endo-activity; Mutants; Functional oligosaccharides
-
Exploiting Systematic Engineering of the Expression Cassette as a Powerful Tool to Enhance Heterologous Gene Expression in Trichoderma reesei
作者:Ji, Wangli;Xu, Xinxin;Zhang, Wei;Xu, Li;Sun, Xianhua;Zhang, Honglian;Luo, Huiying;Yao, Bin;Su, Xiaoyun;Huang, Huoqing
关键词:heterologous gene expression; signal peptide; Kozak sequence; 3 '-UTR; Trichoderma reesei; mannanase