文献类型： 外文期刊

第一作者： Luo, Hengming

作者： Luo, Hengming;Wang, Qun;Guo, Zhixiang;Liu, Lina;He, Chi;Shi, Junyi;Li, Jinbin;Lu, Lin;Dong, Chao;Ma, Qiaoping

作者机构：

关键词： Pi-ta gene; haplotypes; rice landraces; distribution; evolution

期刊名称：GENES （ 影响因子：2.8； 五年影响因子：3.2 ）

ISSN：

年卷期： 2024 年 15 卷 10 期

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收录情况： SCI

摘要： Highlights Distribution of the Pi-ta gene was explored in rice landraces in different regions in Yunnan Province. A total of 385 rice landraces collected from these regions were found to carry the Pi-ta gene. The Pi-ta haplotypes in rice landraces in Yunnan Province were identified and discovered to encode 12 novel Pi-ta protein variations. The evolutionary cluster and network of the Pi-ta haplotypes in rice landraces in Yunnan Province were analyzed. These results suggest that the Pi-ta haplotypes carrying R alleles evolved from S alleles in these rice landraces.Highlights Distribution of the Pi-ta gene was explored in rice landraces in different regions in Yunnan Province. A total of 385 rice landraces collected from these regions were found to carry the Pi-ta gene. The Pi-ta haplotypes in rice landraces in Yunnan Province were identified and discovered to encode 12 novel Pi-ta protein variations. The evolutionary cluster and network of the Pi-ta haplotypes in rice landraces in Yunnan Province were analyzed. These results suggest that the Pi-ta haplotypes carrying R alleles evolved from S alleles in these rice landraces.Highlights Distribution of the Pi-ta gene was explored in rice landraces in different regions in Yunnan Province. A total of 385 rice landraces collected from these regions were found to carry the Pi-ta gene. The Pi-ta haplotypes in rice landraces in Yunnan Province were identified and discovered to encode 12 novel Pi-ta protein variations. The evolutionary cluster and network of the Pi-ta haplotypes in rice landraces in Yunnan Province were analyzed. These results suggest that the Pi-ta haplotypes carrying R alleles evolved from S alleles in these rice landraces.Abstract Background: Rice blast, caused by Magnaporthe oryzae, seriously damages the yield and quality of rice worldwide. Pi-ta is a durable resistance gene that combats M. oryzae carrying AVR-Pita1. However, the distribution of the Pi-ta gene in rice germplasms in Yunnan Province has been inadequately studied. Methods: We analyzed the potential molecular evolution pattern of Pi-ta alleles by examining the diversity in the coding sequence (CDS) among rice varieties. Results: The results revealed that 95% of 405 rice landraces collected from different ecological regions in Yunnan Province carry Pi-ta alleles. We identified 17 nucleotide variation sites in the CDS regions of the Pi-ta gene across 385 rice landraces. These variations led to the identification of 28 Pi-ta haplotypes, encoding 12 novel variants. Among these, 5 Pi-ta haplotypes (62 rice landraces) carried R alleles. The evolutionary cluster and network of the Pi-ta haplotypes suggested that the Pi-ta S alleles were the ancestral alleles, which could potentially evolve into R variants through base substitution. Conclusions: This study suggests that Pi-ta alleles are diverse in the rice landraces in Yunnan, and the Pi-ta sites resistant to blast evolved from the susceptible plants of the rice landraces. These results provide the basis for breeding resistant varieties.

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