Transcriptome Analysis Reveals the Putative Polyketide Synthase Gene Involved in Hispidin Biosynthesis in Sanghuangporus sanghuang

文献类型： 外文期刊

第一作者： Wei, Jiansheng

作者： Wei, Jiansheng;Wei, Jiansheng;Yuan, Xiaolong;Wang, Dong;Wang, Xinyue;Bi, Wei;Wang, Yi;Liu, Liangyan;Wang, Xinyue;Wang, Xinyue;Yang, Yan

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关键词： Sanghuangporus sanghuang; transcriptome; hispidin biosynthesis; polyketide synthase; qRT-PCR

期刊名称：MYCOBIOLOGY （ 影响因子：1.9； 五年影响因子：2.3 ）

ISSN： 1229-8093

年卷期： 2023 年

页码：

收录情况： SCI

摘要： Hispidin is an important styrylpyrone produced by Sanghuangporus sanghuang. To analyze hispidin biosynthesis in S. sanghuang, the transcriptomes of hispidin-producing and non-producing S. sanghuang were determined by Illumina sequencing. Five PKSs were identified using genome annotation. Comparative analysis with the reference transcriptome showed that two PKSs (ShPKS3 and ShPKS4) had low expression levels in four types of media. The gene expression pattern of only ShPKS1 was consistent with the yield variation of hispidin. The combined analyses of gene expression with qPCR and hispidin detection by liquid chromatography-mass spectrometry coupled with ion-trap and time-of-flight technologies (LCMS-IT-TOF) showed that ShPKS1 was involved in hispidin biosynthesis in S. sanghuang. ShPKS1 is a partially reducing PKS gene with extra AMP and ACP domains before the KS domain. The domain architecture of ShPKS1 was AMP-ACP-KS-AT-DH-KR-ACP-ACP. Phylogenetic analysis shows that ShPKS1 and other PKS genes from Hymenochaetaceae form a unique monophyletic clade closely related to the clade containing Agaricales hispidin synthase. Taken together, our data indicate that ShPKS1 is a novel PKS of S. sanghuang involved in hispidin biosynthesis.

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