The Function of Different Subunits of the Molecular Chaperone CCT in the Microsporidium Nosema bombycis: NbCCTζ Interacts with NbCCTα

文献类型： 外文期刊

第一作者： Xu, Sheng

作者： Xu, Sheng;Chen, Ying;Qi, Jingru;Wang, Runpeng;Wei, Erjun;Wang, Qiang;Zhang, Yiling;Tang, Xudong;Shen, Zhongyuan;Wang, Qiang;Zhang, Yiling;Tang, Xudong;Shen, Zhongyuan

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关键词： microsporidia; chaperonin; CCT zeta; coordinating function; immunolocalization

期刊名称：JOURNAL OF FUNGI （ 影响因子：4.7； 五年影响因子：5.2 ）

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年卷期： 2024 年 10 卷 3 期

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收录情况： SCI

摘要： Chaperonin containing tailless complex polypeptide 1 (CCT) is a molecular chaperone protein that consists of eight completely different subunits and assists in the folding of newly synthesized peptides. The zeta subunit of CCT is a regulatory factor for the folding and assembly of cytoskeletal proteins as individuals or complexes. In this study, the zeta subunit of Nosema bombycis (NbCCT zeta) is identified for the first time. The complete ORF of the NbCCT zeta gene is 1533 bp in length and encodes a 510 amino acid polypeptide. IFA results indicate that NbCCT zeta is colocalized with actin and beta-tubulin in the cytoplasm during the proliferative phase and that NbCCT zeta is completely colocalized with NbCCT alpha in the cytoplasm of N. bombycis throughout the entire life cycle. Furthermore, the yeast two-hybrid assay revealed that the NbCCT zeta interacts with NbCCT alpha. The transcriptional level of NbCCT zeta is significantly downregulated by knocking down the NbCCT alpha gene, while the transcriptional level of NbCCT alpha is downregulated after knocking down the NbCCT zeta gene. These results suggest that NbCCT zeta may play a vital role in the proliferation of N. bombycis by coordinating with NbCCT alpha.

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