Formulation of the microbial agent Bacillus amyloliquefaciens NCPSJ7 and its application against Botrytis cinerea in tomato

文献类型： 外文期刊

第一作者： Wang, Junhua

作者： Wang, Junhua;Zhang, Xingwang;Zhang, Yanhao;Qin, Shunxin;Qiu, Jiying;Zhang, Ziyue;Chen, Xiangyan;Chen, Leilei;Zhou, Qingxin;Qin, Shunxin;Zhou, Qingxin;Li, Xihong;Zhou, Qingxin

作者机构：

关键词： Bacillus amyloliquefaciens; Spray drying; Botrytis cinerea; Tomatoes; Microbial agent; Biopesticides

期刊名称：EUROPEAN JOURNAL OF PLANT PATHOLOGY （ 影响因子：1.7； 五年影响因子：1.9 ）

ISSN： 0929-1873

年卷期： 2024 年 169 卷 3 期

页码：

收录情况： SCI

摘要： Spray drying was used to prepare the bacterial powder of Bacillus amyloliquefaciens, which was then used for wettable reagent production. The biocontrol effects of the microbial agent B. amyloliquefaciens against Botrytis cinerea on tomatoes were studied for pre- and postharvest applications. Single-factor analysis was performed to optimize the factors that may affect the bacterial concentration during spray drying, including inlet temperature, feed rate, airflow rate, wall materials, and their concentrations. The parameters were established as follows: inlet temperature of 170 degrees C, feed flow rate of 360 mL/h, airflow rate of 30 m(3)/h, and 10% maltodextrin. The agent with a concentration of 11.30 log colony-forming units (cfu)/g was obtained. The prepared wettable bacterial powder showed significant inhibitory activities against the tested pathogenic fungi, including Fusarium oxysporum, Alternaria alternata, Aspergillus niger, and B. cinerea. After harvest, the tomatoes were sprayed with the prepared microbial agent. The bacterial spores colonized the surface of tomatoes, which prevented B. cinerea from infecting the tomatoes effectively by competition for space and parasitism. The preharvest tomatoes were sprayed with the prepared microbial agent at a dose of 8.1 x 10(7) cfu/mL, which showed an equivalent biocontrol effect to procymidone. Preharvest spray could prolong the storage period and reduce the rotting rate of tomatoes during storage. In conclusion, an efficient and stable B. amyloliquefaciens agent can be prepared by spray drying, which then can be used to control B. cinerea disease pre- and postharvest.

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