Hydrogen administration improves the growth performance of juvenile largemouth bass (Micropterus salmoides) by increasing feed intake, reducing serum lipids, activating mTOR and Nrf2 signaling pathways, and altering the intestinal microbiota

文献类型： 外文期刊

第一作者： Hu, Junru

作者： Hu, Junru;Huang, Wen;Sun, Yuping;Huang, Long;Lai, Mingjian;Li, Xiangni;Wang, Lei;Lin, Yongchun

作者机构：

关键词： Hydrogen gas; Growth performance; mTOR and Nrf2 pathways; Intestinal microbiota; Largemouth bass ( Micropterus salmoides )

期刊名称：AQUACULTURE REPORTS （ 影响因子：3.7； 五年影响因子：3.8 ）

ISSN： 2352-5134

年卷期： 2023 年 33 卷

页码：

收录情况： SCI

摘要： Hydrogen (H-2) gas is recognized for its anti-oxidative, anti-apoptotic, and anti-inflammatory properties in multiple plant and animal models. Nevertheless, there is a lack of research on the effects of H-2 administration on fish. This research seeks to investigate how H-2 administration affects the growth performance, mammalian target of rapamycin (mTOR) and nuclear factor erythroid 2-related factor 2 (Nrf2) signaling pathways, and intestinal microbiota of juvenile largemouth bass under normal feeding conditions. The research randomly allocated 240 fish (3.55 +/- 0.01 g) into two treatment groups, each comprising 4 replicate tanks with 30 fish per tank. The control group was maintained in regular water, while the experimental group (referred to as H(2 )group) was treated with H-2-dissolved water for 1 h per day for 6 weeks. Upon analysis of both groups, it was revealed that the weight gain, final weight, feed intake, specific growth rate, and survival rate were significantly improved by H-2 treatment (P < 0.05). In addition, administration of H2 significantly reduced the triglyceride content and increased the high-density lipoprotein-cholesterol content in the serum (P < 0.05). Moreover, administration of H-2 led to a significant increase in the expression of genes associated with mTOR (PI3K, AKT, TOR, S6K1, and 4EBP1) and Nrf2 (Nrf2, SOD, and GPX) signaling pathways (P < 0.05). Furthermore, H-2 significantly increased the expression of growth hormone receptor (GHR) and neuropeptide Y (NPY) genes in the brain of juvenile largemouth bass. H(2 )did not significantly affect intestinal histology. However, it significantly increased the relative abundance of Sulfuritalea, Bradyrhizobium, and norank_f__Beggiatoaceae (P < 0.05) and reduced the relative abundance of Brevundimonas, unclassified_o__Chlamydiales, and Delftia in the intestinal microbiota (P < 0.05). Thus, these results suggested that providing juvenile largemouth bass with H-2-dissolved water for 1 h per day can lead to a significantly increasing in growth performance, stimulating feed intake, activating mTOR and Nrf2 signaling pathways, and augmenting the abundance of beneficial bacteria in the intestines of juvenile largemouth bass. These findings provided a valuable support for the H-2 application in the largemouth bass healthy culture.

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