PseudoRHDV constructed with feline calicivirus genome as vector has the characteristics of well proliferation in vitro

文献类型： 外文期刊

第一作者： Cheng, Jie

作者： Cheng, Jie;Chen, Jing;Wei, Hulai;Cheng, Jie;Tang, Aoxing;Zhang, Da;Meng, Chunchun;Li, Chuanfeng;Liu, Guangqing

作者机构：

关键词： Felinecali civirus; Rabbit hemorrhagic disease virus; PseudoRHDV

期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.623； 五年影响因子：2.239 ）

ISSN： 0166-0934

年卷期： 2022 年 307 卷

页码：

收录情况： SCI

摘要： Rabbit hemorrhagic disease virus (RHDV) is a major member of the Caliciviridae. which is fatal to wild and domestic European rabbit. Because RHDV does not reproduce stably in vitro, molecular studies on this pathogen have been limited. Feline calicivirus (FCV), also a member of the Caliciviridae, reproduces well in vitro and is a good viral vector. As these viruses share similar genomic structures, we hypothesized that a chimeric infectious clone could be constructed by replacing the corresponding regions of the FCV genome with the structural proteins VP60 and VP10 and the 3 & PRIME; non-translated region of the RHDV genome. Transfection of the infectious clone into RK13 cells made it possible to rescue the chimeric virus, named pseudoRHDV, which reproduced in an RK13 cell line with high titer. An infectious pseudoRHDV was produced, which proliferated in RK13 cells to at least 15 generations. PseudoRHDV caused significant cytopathic changes in the RK13 cells, with a viral titer was 9.74 log10 TCID50/mL. The pseudoRHDV constructed in this study will be helpful for investigating the molecular biology of RHDV, especially its interaction with the host. The model can also be used to explore some common laws between FCV and RHDV.

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