Mitochondrial gene expression analysis reveals aberrant transcription of cox3 in Gossypium barbadense CMS line H276A
文献类型： 外文期刊
第一作者： Khan, Aziz
作者： Khan, Aziz;Kong, Xiangjun;Liao, Xiaofang;Zheng, Jie;You, Jingyi;Li, Min;Raza, Haneef;Zhou, Ruiyang;Zheng, Jie;Hussain, Reem M.
作者机构：
关键词： Cotton (Gossypium barbadense); Cytoplasmic male sterility; Mitochondrial genes; Circularized RNA reverse-transcribed PCR
期刊名称：DEVELOPMENT GENES AND EVOLUTION （ 影响因子：2.116； 五年影响因子：2.672 ）
ISSN： 0949-944X
年卷期： 2022 年 232 卷 1 期
页码：
收录情况： SCI
摘要： Cotton cytoplasmic male sterility (CMS) has been extensively studied; however, information regarding its molecular mechanisms has not yet been disclosed. Therefore, to explore the molecular mechanism of pollen abortion of cotton CMS line H276A, transcript profiles of 30 mitochondrial protein-encoding genes at tetrad stage were conducted with northern blot and a differential expression gene cox3 was identified. Quantitative reverse-transcribed PCR (qRT-PCR) analysis indicated that the expression level of cox3 in the CMS line H276A was 0.39-fold compared to its maintainer line H276B. In addition, the immunoblot analysis revealed that the amount of COX3 protein was decreased to 59.38% in CMS line H276A. The 5` and 3` terminals of the transcript of cox3 in two materials were determined simultaneously with circularized RNA reverse-transcribed PCR (CR-RT-PCR). The data indicated that seven 5` end of transcript of cox3 in H276A (-451/-464/-465/-467/-471/-472/-508 respect to ATG) were identified which were different from that of H276B (-411/-412). A total of 15 single nucleotide polymorphisms (SNPs) was detected by clone sequencing analysis of upstream of cox3. To our knowledge, we are the first to comprehensively analyze the transcripts of the mitochondrial genome in the cotton CMS line and to identify the 5` and 3` terminals of the transcript of cox3 in cotton. Our data will provide a framework for a better understanding of molecular mechanisms of CMS and mitochondrial gene expression in cotton.
分类号：
- 相关文献
作者其他论文 更多>>
-
Multiplex one-step RT-qPCR assays for simultaneous detection of BRV, BCoV, Escherichia coli K99+ and Cryptosporidium parvum
作者：Zhao, Xinru;Li, Min;Yang, Yingying;Wang, Yidan;Zheng, Xiaoru;Li, Huatao;Fu, Kaiqiang;Cao, Zhi;Yin, Dehua;Gao, Haihui
关键词：BRV; BCoV;
E. coli K99(+);C. parvum ; multiplexTaq Man real-time PCR -
IPM-AgriGPT: A Large Language Model for Pest and Disease Management with a G-EA Framework and Agricultural Contextual Reasoning
作者：Zhang, Yuqin;Fan, Qijie;Chen, Xuan;Li, Fuzhong;Guo, Leifeng;Zhang, Yuqin;Fan, Qijie;Chen, Xuan;Li, Min;Guo, Leifeng;Zhao, Zeying
关键词：large language models; pest and disease; chain-of-thought distillation; agriculture
-
Analysis of the Transcriptome Provides Insights into the Photosynthate of Maize Response to Salt Stress by 5-Aminolevulinic Acid
作者：Jiang, Ying;Li, Min;Qian, Yumei;Rong, Hao;Xie, Tao;Wang, Shanshan;Zhao, Hong;Yang, Liangli;Wang, Qingyun;Cao, Yanyong
关键词：salt stress;
Zea mays L.; 5-aminolevulinic acid; photosynthesis; transcriptome -
Molecular Cloning and Heterologous Expression of the Mitochondrial ATP6 Gene from Kenaf (Hibiscus cannabinus) in Tobacco (Nicotiana tabacum)
作者：Huang, Bangbang;Zhou, Ruiyang;Wei, Rongchang;Hou, Wenhuan;Tang, Xingfu;Zhao, Yanhong;Liao, Xiaofang;Wei, Meiling
关键词：kenaf; cytoplasmic male sterility;
atp6 ; construction of overexpression vectors; transformation -
Molecular mechanism of potently neutralizing human monoclonal antibodies against severe fever with thrombocytopenia virus infection
作者：Quan, Chuansong;Nie, Kaixiao;Zheng, Wenjun;Wang, Yiwen;Quan, Chuansong;Zhang, Hong;Quan, Chuansong;Ma, Dezhen;Su, Chao;Li, Lianfeng;Peng, Dingkun;Yin, Chunhong;Yang, Peipei;Shan, Chao;Liu, Xin;Zheng, Jie;Shi, Weifeng;Li, Weiwei;Gao, George F.;Qi, Jianxun;Liu, Weixiao;Liu, Di;Carr, Michael J.;Carr, Michael J.;Shi, Weifeng
关键词：severe fever with thrombocytopenia syndrome; SFTSV; Bandavirus dabieense; monoclonal antibodies; neutralization; therapeutics
-
Resistance risk and mechanism of prochloraz in Fusarium solani
作者：Mao, Xuewei;Cao, Tangbo;Chen, Liting;Li, Min;Zhao, Xingchen;Liu, Hao;Wang, Li;Zhou, Lin;Mao, Xuewei;Wang, Li;Zhou, Lin;Mao, Xuewei;Wang, Li;Zhou, Lin;Mao, Xuewei;Duan, Yun
关键词：Fusarium solani; Prochloraz; Baseline sensitivity; Resistance mechanisms; Resistance management
-
A comprehensive analysis of the O-methyltransferase gene family in the chili pepper (Capsicum annuum) identifies COMT36 involved in capsaicinoids biosynthesis
作者：Li, Shanglin;Cai, Yutong;Yang, Ruyi;Chen, Guoju;Chen, Changming;Zhu, Zhangsheng;Ma, Hongmei;Liu, Yixuan;Zheng, Jie;Zhou, Xin;He, Jianwen;Wu, Di
关键词：Capsicum; O-methyltransferase; Gene family; Capsaicin
