A Smart Single-Loop-Mediated Isothermal Amplification Facilitates Flexible SNP Probe Design for On-Site Rapid Differentiation of SARS-CoV-2 Omicron Variants

文献类型： 外文期刊

第一作者： Lin, Qijie

作者： Lin, Qijie;Qu, Xiaoyun;Deng, Yuhui;Cai, Qianyi;Liang, Yucen;Xu, Xiaozhen;Liu, Ruidong;Zhang, Jianmin;Liao, Ming;Gou, Hongchao;Du, Shouwen;Jia, Kaiyuan;Lin, Jianhan;Li, Dan;Yan, Bailing;Li, Yanbin;Li, Letian;Jiang, Yuhang;Deng, Lingcong;Li, Chang;Liao, Ming

作者机构：

关键词： nucleic acid amplification; SARS-CoV-2 variants of concern; SNP detection; ssLAMP

期刊名称：ADVANCED SCIENCE （ 影响因子：14.1； 五年影响因子：15.6 ）

ISSN：

年卷期： 2025 年 12 卷 26 期

页码：

收录情况： SCI

摘要： Rapid on-site typing methods for SARS-CoV-2 variants of concern are crucial for its effective surveillance and control. Herein, a smart single-loop-mediated isothermal amplification (ssLAMP) method with the absence of an inner primer but the addition of a swarm primer for differentiation of SARS-CoV-2 Omicron variants is developed. This unique primer design strategy offers greater flexibility in introducing single nucleotide polymorphism (SNP) identification probes and enables multiple detection assays for SARS-CoV-2 Omicron variants including BA.1, BA.2, BA.3, BA.4, and BA.5. A 3D-printed portable dual fluorescence visualization device and smartphone app are developed to enable point-of-care testing. This assay is rapid (within 90 min), highly sensitive (100 copies/reaction), and specific (identification of SNP) for SARA-CoV-2 Omicron variants. The ssLAMP method identifies five BA.5-positive samples among 97 nasopharyngeal swab samples from the clinic, with a 100% concordance rate with Sanger sequencing. The ssLAMP assay system is expected to be utilized for on-site, highly specific, and rapid visualization detection of SARS-CoV-2 and its variants, with great application potential in pathogen genotyping, early cancer screening, and other areas of SNP mutation detection.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序