Genome-wide assessment of genetic variation and differentiation for Gastrodia elata germplasm based on SLAF sequencing
文献类型： 外文期刊
第一作者： Xu, Zhe
作者： Xu, Zhe;Zhang, Lei;Qian, Huali;Chen, Xiaolei;Su, Jianyun;Chen, Hao;Dong, Jiahong;Ji, Pengzhang;Xu, Zhe;Zhang, Lei;Qian, Huali;Chen, Xiaolei;Su, Jianyun;Chen, Hao;Dong, Jiahong;Ji, Pengzhang;Shi, Yana;Cong, Kun
作者机构：
关键词： Gastrodia elata BI; Genetic diversity; SLAF-seq SNP
期刊名称：GENETIC RESOURCES AND CROP EVOLUTION （ 影响因子：2.0； 五年影响因子：1.9 ）
ISSN： 0925-9864
年卷期： 2023 年 70 卷 7 期
页码：
收录情况： SCI
摘要： Gastrodia elata BI. is an important cultivated medicinal plant in China. To analyze the genetic diversity and evolutionary relationship of the germplasm resources of G. elata, SNP locus and SNP typing of G. elata accessions were identified by specific-locus amplified fragment (SLAF) sequencing. SLAF analysis was used to compare 28 samples of the same G. elata cultivar. Plants of three different varieties from four different populations were collected to explore intraspecific variation and to lay a foundation for resource protection and thus improved variety breeding. In this study, genetic variation and differentiation of G. elata f. glauca, G. elata f. viridis, and G. elata f. elata were analyzed using specific-locus amplified fragment (SLAF) sequencing. A total of 75.95 M reads with an average sequencing depth of 19.32 x and a mean Q30 of 91.71% were obtained. Based on the 19,675 polymorphic SLAF tags, 60,238 SNPs were identified, and a subset of 22,737 SNPs with minor allele frequency > 0.05 and integrity > 0.5 was selected for further analysis. A model-based analysis divided the accessions into two groups, wild-type G. elata f. glauca and G. elata f. viridis groups. Phylogenetic analysis also clustered the samples into the same two major groups. G. elata has high genetic diversity. Population diversity was highest in G. elata f. elata and lowest in G. elata f. viridis. Analysis of molecular variance (AMOVA) revealed significant polymorphism within individuals (92.23%). This study provides new insights into the genetic variation and differentiation of G. elata, which can be exploited to improve existing commercial cultivars.
分类号：
- 相关文献
作者其他论文 更多>>
-
Characterization of rhizosphere bacterial communities in oilseed rape cultivars with different susceptibility to Plasmodiophora brassicae infection
作者：Deng, Yue;Huang, Xiaoqing;Yang, Xiaoxiang;Yu, Yaoyin;Zhang, Zhongmei;Hu, Zijin;Zhou, Xiquan;Liu, Yong;Zhang, Lei;Deng, Yue;Wu, Wenxian;Huang, Xiaoqing;Yang, Xiaoxiang;Yu, Yaoyin;Zhang, Zhongmei;Liu, Yong;Zhang, Lei;Zhou, Kang;Zhou, Kang
关键词：rhizosphere microbiome;
Plasmodiophora brassicae ; susceptible cultivar; resistant cultivar; microbial metabolism; nitrogen cycle -
Biodegradable film drip fertigation is more conducive to reducing the diversity and abundance of antibiotic resistance genes than plastic film drip fertigation
作者：Li, Yingjie;Yuan, Yuhan;Li, Cuilan;Zhang, Jinjing;Liu, Hang;Wang, Meng;Li, Qian;Zhang, Lei;Sun, Bo;Qin, Yubo
关键词：Antibiotic resistance genes; Biodegradable film drip fertigation; Bacterial diversity; HT-PCR
-
Para-coumaric acid and cinnamic acid enhance resistance of Agaricus bisporus s mushrooms to Brown blotch disease caused by Pseudomonas tolaasii
作者：Shi, Zixuan;Song, Rui;Zhang, Lei;Jiang, Hanyue;Jiao, Lu;Yuan, Shuai;Meng, Demei;Zheng, Yanyan;Chen, Lin
关键词：Bacterial disease; Edible mushroom; Induced resistance; Phenolic acid; Storage
-
Jatrophane diterpenoids from Jatropha curcas with multidrug resistance reversal activity
作者：He, Yi-Lin;Li, Guo-Li;Yang, Hong-Ying;Zhang, Lei;Gong, Zheng;He, Yi-Lin;Zhang, Lan-Jun;Yang, Hong-Ying;Gao, Kun;Zhang, Lan-Jun
关键词：Jatropha curcas L.; Euphorbiaceae; Jatrophane diterpenoids; Multidrug resistance reversal activities
-
Black soybean peptide mediates the AMPK/SIRT1/NF-κB signaling pathway to alleviate Alzheimer's-related neuroinflammation in lead-exposed HT22 cells
作者：Yang, Huijie;Zhao, Peijun;Ullah, Saif;Ma, Yan;Zhao, Guangshan;Cheng, Yongxia;Li, Qian;Li, Tiange;Qiao, Mingwu;Song, Lianjun;Zhang, Lei;Huang, Xianqing;Li, Ning;Wang, Fangyu;Galaverna, Gianni
关键词：Black soybean peptide; Lead (Pb); Alzheimer's disease; Neuroinflammation; AMPK/SIRT1/NF-kappa B pathway
-
Diversity and distribution of Mdace mutations involved in propoxur resistance in the house fly (Musca domestica L.) in China
作者：You, Chunmei;Ji, Xiangyun;You, Chunmei;Zhang, Lulu;Song, Jiajia;Zhen, Congai;Zhang, Lei;Gao, Xiwu
关键词：Musca domestica; propoxur; acetylcholinesterase; Mdace; mutation
-
Risk Assessment of Cyclaniliprole Resistance in Peach-Potato Aphid Myzus persicae: Laboratory Selection, Inheritance, and Cross-Resistance Patterns
作者：Liang, Pingzhuo;Zhang, Zihao;Zhen, Congai;Zhang, Lei;Li, Dapeng;Cheng, Shenhang;Li, Ren
关键词：cross-resistance; cyclaniliprole; inheritance;
Myzus persicae ; reproduction fitness; resistance selection; risk assessment
