Biogenic Selenium Nanoparticles Synthesized by L. brevis 23017 Enhance Aluminum Adjuvanticity and Make Up for its Disadvantage in Mice

文献类型： 外文期刊

第一作者： Zhang, Zheng

作者： Zhang, Zheng;De, Xinqi;Sun, Weijiao;Li, Yifan;Yang, Zaixing;Liu, Ning;Wu, Jingyi;Miao, Yaxin;Wang, Jiaqi;Ge, Junwei;Liu, Runhang;Wang, Fang;Ge, Junwei

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关键词： Levilactobacillus brevis; Selenium nanoparticles; Aluminum adjuvants; Immunoenhancement; SIgA antibody

期刊名称：BIOLOGICAL TRACE ELEMENT RESEARCH （ 影响因子：3.9； 五年影响因子：3.8 ）

ISSN： 0163-4984

年卷期： 2024 年

页码：

收录情况： SCI

摘要： The most popular vaccine adjuvants are aluminum ones, which have significantly reduced the incidence and mortality of many diseases. However, aluminum-adjuvanted vaccines are constrained by their limited capacity to elicit cellular and mucosal immune responses, thus constraining their broader utilization. Biogenic selenium nanoparticles are a low-cost, environmentally friendly, low-toxicity, and highly bioactive form of selenium supplementation. Here, we purified selenium nanoparticles synthesized by Levilactobacillus brevis 23017 (L-SeNP) and characterized them using Fourier-transform infrared spectroscopy, energy-dispersive X-ray spectroscopy, scanning electron microscopy, and transmission electron microscopy. The results indicate that the L-SeNP has a particle size ranging from 30 to 200 nm and is coated with proteins and polysaccharides. Subsequently, we assessed the immune-enhancing properties of L-SeNP in combination with an adjuvant-inactivated Clostridium perfringens type A vaccine using a mouse model. The findings demonstrate that L-SeNP can elevate the IgG and SIgA titers in immunized mice and modulate the Th1/Th2 immune response, thereby enhancing the protective effect of aluminum-adjuvanted vaccines. Furthermore, we observed that L-SeNP increases selenoprotein expression and regulates oxidative stress in immunized mice, which may be how L-SeNP regulates immunity. In conclusion, L-SeNP has the potential to augment the immune response of aluminum adjuvant vaccines and compensate for their limitations in eliciting Th1 and mucosal immune responses.

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