Nucleolin interacts with the rabbit hemorrhagic disease virus replicase RdRp, nonstructural proteins p16 and p23, playing a role in virus replication

文献类型： 外文期刊

第一作者： Zhu, Jie

作者： Zhu, Jie;Miao, Qiuhong;Guo, Hongyuan;Tang, Aoxing;Dong, Dandan;Tang, Jingyu;Tong, Guangzhi;Liu, Guangqing;Miao, Qiuhong;Wang, Fang

作者机构：

关键词： Rabbit hemorrhagic disease virus (RHDV); Nucleolin; Virus replication; RNA-dependent RNA polymerase (RdRp)

期刊名称：VIROLOGICA SINICA （ 影响因子：6.947； 五年影响因子：5.997 ）

ISSN： 1674-0769

年卷期： 2022 年 37 卷 1 期

页码：

收录情况： SCI

摘要： Rabbit hemorrhagic disease virus (RHDV) is a member of the Caliciviridae family and cannot be propagated in vitro, which has impeded the progress of investigating its replication mechanism. Construction of an RHDV replicon system has recently provided a platform for exploring RHDV replication in host cells. Here, aided by this replicon system and using two-step affinity purification, we purified the RHDV replicase and identified its associated host factors. We identified rabbit nucleolin (NCL) as a physical link, which mediating the interaction between other RNA-dependent RNA polymerase (RdRp)-related host proteins and the viral replicase RdRp. We found that the overexpression or knockdown of NCL significantly increased or severely impaired RHDV replication in RK-13 cells, respectively. NCL was identified to directly interact with RHDV RdRp, p16, and p23. Furthermore, NCL knockdown severely impaired the binding of RdRp to RdRp-related host factors. Collectively, these results indicate that the host protein NCL is essential for RHDV replication and acts as a physical link between viral replicase and host proteins.

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[1]Nucleolin interacts with the rabbit hemorrhagic disease virus replicase RdRp, nonstructural proteins p16 and p23, playing a role in virus replication. Zhu, Jie,Miao, Qiuhong,Guo, Hongyuan,Tang, Aoxing,Dong, Dandan,Tang, Jingyu,Tong, Guangzhi,Liu, Guangqing,Miao, Qiuhong,Wang, Fang. 2022

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