Transcriptomic and metabolomic analyses reveal that exogenous strigolactones alleviate the response of melon root to cadmium stress

文献类型： 外文期刊

第一作者： Chen, Xuemiao

作者： Chen, Xuemiao;Shi, Xueyin;Ai, Qing;Han, Jinying;Wang, Huaisong;Fu, Qiushi

作者机构：

关键词： Melon; Strigolactones; Cd stress; Root; Transcriptome; Metabolome

期刊名称：HORTICULTURAL PLANT JOURNAL （ 影响因子：4.24； ）

ISSN： 2095-9885

年卷期： 2022 年 8 卷 5 期

页码：

收录情况： SCI

摘要： Strigolactones (SLs) are classified into plant hormones, playing a key role as a mediator of plant growth in response to several abiotic stresses. Cadmium (Cd), a common heavy metal and soil pollutant, can suppress plant growth and development. In this work, we explored the effects of exogenous SLs on root formation in response to Cd stress using melon seeds subjected to seven germination treatments: CK (control), Cd (300 mu mol.L-1 CdCl2), and SL1eSL5 (CdCl2-stressed seeds pretreated with 0.1, 0.5, 1, 2, and 3 mu mol.L-1 GR24 solutions). The results indicated that SLs increased the antioxidant enzyme activities and root vigor and decreased the malondialdehyde (MDA) contents in the roots of Cd-stressed melon seedlings. Then we used transcriptomic and metabolomic analyses to explore the mechanisms by which exogenous SLs protect against Cd stress. There were 242 significant differentially expressed genes (DEGs) (78 upregulated, 164 downregulated) and 247 significantly differentially expressed metabolites (DEMs) (222 upregulated, 25 downregulated) between the Cd and SL3 treatments. SLs altered the expression of genes related to redox formation processes, including peroxidase (POD), lipoxygenase (LOX), glutamate dehydrogenase (GDH), and glutathione S-transferase (GST). In addition, we found that SLs regulated the expression of the MYB, AP2/ERF, bHLH, and WRKY tran-scription factor families. The combined transcriptomic and metabolomic analyses revealed that the DEGs and DEMs involved in Cd stress alleviation were mainly related to the gene expression of jasmonic acid (JA) and flavonoid biosynthesis. SLs might induce LOX-related genes to regulate JA biosynthesis. Moreover, SLs might promote flavonoid biosynthesis by regulating eleven flavonoid-related genes and eight me-tabolites. The results provide a new perspective for studying the adaptation of plants to Cd stress.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序