Heterologous Expression of Sunflower HaHPT and HaTMT Genes Enhances Rice-Grain Vitamin E Content

文献类型： 外文期刊

第一作者： Song, Shuang

作者： Song, Shuang;Li, Hang;Lin, Shaoyan;Dong, Xiaoou;Tian, Ruiping;Wu, Zewan;Li, Qing;Li, Mingyi;Zhang, Keying;Liu, Xi;Wan, Jianmin;Liu, Linglong;Dong, Xiaoou;Liu, Xi;Wan, Jianmin;Liu, Linglong;Wan, Jianmin

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关键词： rice (Oryza sativa L.); vitamin E; sunflower (Helianthus annuus L.); homogentisate phytyltransferase; gamma-tocopherol methyltransferase

期刊名称：PLANTS-BASEL （ 影响因子：4.1； 五年影响因子：4.5 ）

ISSN： 2223-7747

年卷期： 2024 年 13 卷 17 期

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收录情况： SCI

摘要： Insufficient dietary vitamin intake can lead to severe health conditions in humans. Improving the vitamin E (VE) content of food crops such as rice through breeding is an economical and effective means to alleviate this problem. In this study, Homogentisate phytyltransferase (HPT) and gamma-tocopherol methyltransferase (gamma-TMT), two genes derived from sunflower (Helianthus annuus L., a high VE species), were introduced into an elite rice (Oryza sativa L.) cultivar "Ningjing 7" for biofortification. We verified the successful expression of the two genes in multiple transformation events. High-performance liquid chromatography revealed that transgenic plants expressing either HaHPT alone or HaHPT and HaTMT accumulate more VE compared with the wild type. We also revealed that the level of alpha-tocopherol, the form of VE with the highest biological activity, had increased to 2.33 times in transgenic HaTMT plants compared with the wild type. Transcriptome analysis revealed that the expression levels of some chlorophyll synthesis pathway genes related to VE precursor synthesis significantly increased during grain filling in transgenic rice grains. No difference in agronomic traits was observed between the transgenic plants and their wild type except for a slightly reduced plant height associated with the transgenic plants. These data demonstrate that the heterologous expression of HaHPT gene is effective in increasing the total VE content, while HaTMT plays an important role in the relative abundance of alpha-tocopherol in rice grains. This study demonstrates a promising strategy for breeding rice with elevated VE content via metabolic engineering.

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