Functional analysis of all succinate dehydrogenase subunits in Fusarium fujikuroi

文献类型： 外文期刊

第一作者： Fan, Mengyuan

作者： Fan, Mengyuan;Qi, Hao;Shao, Wenyong;Yin, Yanni;Chen, Yun;Ma, Zhonghua;Zhang, Hao;Zhao, Youfu

作者机构：

关键词： Fusarium fujikuroi; Rice bakanae disease; Succinate dehydrogenase inhibitors (SDHIs); Succinate dehydrogenase (Sdh) subunit

期刊名称：PHYTOPATHOLOGY RESEARCH （ 影响因子：3.2； 五年影响因子：3.7 ）

ISSN： 2096-5362

年卷期： 2024 年 6 卷 1 期

页码：

收录情况： SCI

摘要： Fusarium fujikuroi, the causal agent of rice bakanae disease (RBD), contains five succinate dehydrogenase (Sdh) subunits: FfSdhA, FfSdhB, FfSdhC1, FfSdhC2, and FfSdhD. However, the role of these subunits in regulating sensitivity to succinate dehydrogenase inhibitors (SDHIs) is largely unknown. Here, we conducted targeted gene disruption and phenotypic assays for all Sdh subunits and found that the deletion mutants of FfSdhA, FfSdhB, and FfSdhD exhibited severe defects in hyphal growth, conidiation, virulence, and sensitivity to CaCl2 and oxidative stresses. To a lesser extent, the mycelial growth rate and conidial production of Delta FfSdhC1 were also decreased as compared to those of the wild-type strain JS16. In addition, fungicide sensitivity assays showed that deletion of FfSdhA, B, C1, or D led to decreased sensitivity to all SDHIs tested. Unexpectedly, we were unable to obtain a FfSdhC1 + C2 double mutant and further found significant up-regulation of FfSdhC2 in triangle FfSdhC1, indicating that FfSdhC1 and -C2 might be essential for fungal growth although the FfSdhC2 deletion mutant was indistinguishable from the wild-type strain. These findings provide useful information for enhancing our understanding of the biological functions of the Sdh subunits in pathogenic fungi.

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