MiR-23b and miR-133 Cotarget TGFβ2/NOTCH1 in Sheep Dermal Fibroblasts, Affecting Hair Follicle Development

文献类型： 外文期刊

第一作者： He, Junmin

作者： He, Junmin;Wei, Chen;Zhang, Guoping;Mao, Jingyi;Tian, Kechuan;Liu, Guifen;He, Junmin;Wei, Chen;Zhang, Guoping;Tian, Kechuan;Liu, Guifen;Huang, Xixia;Li, Xue;Yang, Cunming;Zhang, Wenjing

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关键词： hair follicle development; sheep dermal fibroblasts; cotargeting; proliferation; migrate; apoptosis

期刊名称：CELLS （ 影响因子：6.0； 五年影响因子：6.7 ）

ISSN：

年卷期： 2024 年 13 卷 6 期

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收录情况： SCI

摘要： Wool is produced and controlled by hair follicles (HFs). However, little is known about the mechanisms involved in HF development and regulation. Sheep dermal fibroblasts (SDFs) play a key role in the initial stage of HF development. Analyzing the molecular mechanism that regulates early HF development in superfine wool sheep is of great importance for better understanding the HF morphogenesis process and for the breeding of fine wool sheep. Here, we show that two microRNAs (miRNAs) affect the development of HFs by targeting two genes that are expressed by SDFs. Meanwhile, the overexpression and inhibition of oar-miR-23b and oar-miR-133 in SDFs cells and cell proliferation, apoptosis, and migration were further detected using a CCK-8 assay, an Annexin V-FITC assay, a Transwell assay, and flow cytometry. We found that oar-miR-23b, oar-miR-133, and their cotarget genes TGF beta 2 and NOTCH1 were differentially expressed during the six stages of HF development in superfine wool sheep. Oar-miR-23b and oar-miR-133 inhibited the proliferation and migration of SDFs and promoted the apoptosis of SDFs through TGF beta 2 and NOTCH1. oar-miR-23b and oar-miR-133 inhibited the proliferation and migration of SDFs by jointly targeting TGF beta 2 and NOTCH1, thereby inhibiting the development of superfine wool HFs. Our research provides a molecular marker that can be used to guide the breeding of ultrafine wool sheep.

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