Selection and Validation of Reference Genes in Virus-Infected Sweet Potato Plants

文献类型： 外文期刊

第一作者： Li, Guangyan

作者： Li, Guangyan;Sun, Xiaohui;Wu, Bin;Hong, Hao;Xin, Zhimei;Xin, Xiangqi;Jiang, Shanshan;Zhu, Xiaoping;Peng, Jiejun;Jiang, Shanshan

作者机构：

关键词： expression stability; quantitative real-time PCR; reference genes; experimental conditions

期刊名称：GENES （ 影响因子：3.5； 五年影响因子：3.9 ）

ISSN：

年卷期： 2023 年 14 卷 7 期

页码：

收录情况： SCI

摘要： Quantitative real-time PCR (qRT-PCR) in sweet potatoes requires accurate data normalization; however, there are insufficient studies on appropriate reference genes for gene expression analysis. We examined variations in the expression of eight candidate reference genes in the leaf and root tissues of sweet potatoes (eight nonvirus-infected or eight virus-infected samples). Parallel analyses with geNorm, NormFinder, and Best-Keeper show that different viral infections and origin tissues affect the expression levels of these genes. Based on the results of the evaluation of the three software, the adenosine diphosphate-ribosylation factor is suitable for nonvirus or virus-infected sweet potato leaves. Cyclophilin and ubiquitin extension proteins are suitable for nonvirus-infected sweet potato leaves. Phospholipase D1 alpha is suitable for virus-infected sweet potato leaves. Actin is suitable for roots of nonvirus-infected sweet potatoes. Glyceraldehyde-3-phosphate dehydrogenase is suitable for virus-infected sweet potato roots. The research provides appropriate reference genes for further analysis in leaf and root samples of viruses in sweet potatoes.

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