NLRs derepress MED10b-and MED7-mediated repression of jasmonate- dependent transcription to activate immunity

文献类型： 外文期刊

第一作者： Wu, Qian

作者： Wu, Qian;Tong, Cong;Chen, Zhengqiang;Huang, Shen;Hong, Hao;Li, Jia;Feng, Mingfeng;Wang, Huiyuan;Xu, Min;Yan, Yuling;Cui, Hongmin;Shen, Danyu;Ai, Gan;Xu, Yi;Dong, Suomeng;Wang, Xuan;Zhu, Min;Tao, Xiaorong;Zhao, Xiaohui;Wang, Huiyuan;Li, Junming;Zhang, Hui;Huang, Changjun;Zhang, Zhongkai;Dinesh-Kumar, Savithramma P.;Dinesh-Kumar, Savithramma P.

作者机构：

关键词： Mediator complex; NLRs; Sw-5b; effector-triggered immunity

期刊名称：PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA （ 影响因子：11.1； 五年影响因子：12.0 ）

ISSN： 0027-8424

年卷期： 2023 年 120 卷 28 期

页码：

收录情况： SCI

摘要： Plant intracellular nucleotide-binding domain, leucine- rich repeat-containing receptors (NLRs) activate a robust immune response upon detection of pathogen effectors. How NLRs induce downstream immune defense genes remains poorly understood. The Mediator complex plays a central role in transducing signals from gene-specific transcription factors to the transcription machinery for gene transcription/activation. In this study, we demonstrate that MED10b and MED7 of the Mediator complex mediate jasmonate-dependent transcription repression, and coiled coil NLRs (CNLs) in Solanaceae modulate MED10b/MED7 to activate immunity. Using the tomato CNL Sw-5b, which confers resistance to tospovirus, as a model, we found that the CC domain of Sw- 5b directly interacts with MED10b. Knockout/down of MED10b and other subunits including MED7 of the middle module of Mediator activates plant defense against tospovirus. MED10b was found to directly interact with MED7, and MED7 directly interacts with JAZ proteins, which function as transcriptional repressors of jasmonic acid (JA) signaling. MED10b-MED7-JAZ together can strongly repress the expression of JA-responsive genes. The activated Sw- 5b CC interferes with the interaction between MED10b and MED7, leading to the activation of JA-dependent defense signaling against tospovirus. Furthermore, we found that CC domains of various other CNLs including helper NLR NRCs from Solanaceae modulate MED10b/MED7 to activate defense against different pathogens. Together, our findings reveal that MED10b/ MED7 serve as a previously unknown repressor of jasmonate-dependent transcription repression and are modulated by diverse CNLs in Solanaceae to activate the JA-specific defense pathways.

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