The effects of selenium on the translation efficiency of selenoproteins GPX1, GPX4, and TXNRD1 in oxLDL-induced EA.hy926 cells

文献类型： 外文期刊

第一作者： Lu, Jiaqi

作者： Lu, Jiaqi;Meng, Jing;Lang, Yanzhi;Ran, Linwu;Rehman, Abdul;Yan, Yamei

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关键词： Selenium; Oxidized low density lipoprotein (oxLDL); Selenoprotein; Ribosome profiling; Translational efficiency

期刊名称：FOOD SCIENCE AND HUMAN WELLNESS （ 影响因子：7.4； 五年影响因子：7.8 ）

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年卷期： 2025 年 14 卷 1 期

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收录情况： SCI

摘要： Selenium is a crucial trace element that contributes to physiological processes in the body as selenoproteins. Selenoproteins serve as an integral role in the body in controlling the redox state of cells and protecting against damage induced by oxidative stress. This study aimed to investigate the effects and possible mechanism of selenium on selenoproteins expression in EA.hy926 cells induced by oxidized low density lipoprotein (oxLDL). The impact of selenium on the viability of EA.hy926 cells was detected by the methylthiazolyldiphenyltetrazolium bromide (MTT) method, and intracellular reactive oxygen species (ROS) level and mitochondrial membrane potential were assessed by fluorescent probe DCFH-DA and JC-1, respectively. RNA-seq, quantitative real-time polymerase chain reaction (qPCR), and Western blot were used to investigate the selenoprotein expression. Selenoprotein mRNA translation efficiency was analyzed by ribosome profiling (Ribo-Seq) coupled with transcriptomics. Our data showed that selenium supplementation (0.5 mu mol/L) significantly decreased ROS production, increased mitochondrial inner membrane potential and increased the proliferative activity of EA.hy926 cells induced by oxLDL. Moreover, The protective effects of selenium against oxLDL-induced EA.hy926 cell injury were associated with the upregulation of the expressions of selenoproteins glutathione peroxidase 1 (GPX1), glutathione peroxidase 4 (GPX4), and thioredoxin reductase 1 (TXNRD1). Furthermore, the expressions of selenoproteins GPX1 and GPX4 were hierarchically controlled, but the expressions of selenoproteins TXNRD1 were mainly regulated by oxLDL. Finally, Ribo-Seq coupled with transcriptomics results demonstrated that the expressions of selenoproteins GPX1, GPX4, and TXNRD1 were regulated at the translation process level. These findings suggested that selenium could have preventive effects in oxLDL induced EA.hy926 cell injury by regulating the selenoprotein expression, and the selenoproteins expressions at the translation level in vascular endothelial cells need further study. (c) 2025 Beijing Academy of Food Sciences. Publishing services by Tsinghua University Press. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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