文献类型： 外文期刊
第一作者： Han, Zongchen
作者： Han, Zongchen;Niu, Chenqi;Huang, Kuo;Ye, Changwen;Hao, Jie;Sun, Zhikang;Zhang, Zekun;Zou, Dian;Wei, Xuetuan;Lu, Qi
作者机构：
关键词：
tobacco starch;
期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：4.5； 五年影响因子：5.2 ）
ISSN：
年卷期： 2025 年 16 卷
页码：
收录情况： SCI
摘要： The combustion of excessive starch in tobacco leaves leads to more harmful substances, adversely affecting the sensory properties of tobacco and posing significant risks to human health. Therefore, there is an urgent need to develop specific amylases targeting tobacco starch to address these issues. In this study, 5 different alpha-amylase genes were selected for recombinant expression in Bacillus amyloliquefaciens BAX-5, and the amyA(LC) (derived from Bacillus amyloliquefaciens MK10163) was confirmed to be the optimal gene. Then, the alpha-amylase activity was further increased by screening host bacteria BAX-5 and signal peptides SP003 (derived from the dacB gene of Bacillus subtilis 168). Subsequently, the alpha-amylase properties were characterized, such as temperature tolerance, pH tolerance and metal ion. Through replacement of culture medium, the recombinant strain BAX-5/PT-17SP003amyA(LC) produced the maximum alpha-amylase activity of 904.91 IU/mL, which was about 4 times higher than that of the original culture medium. Finally, the alpha-amylase Amy (LC) was applied to the enzyme treatment of tobacco leaves, and the evaluation results showed that alpha-amylase Amy (LC) could play a positive role in reducing damage and enhancing quality of cigarettes. This research provides a novel enzymatic resource for the development of amylases, and it has enormous market potential and application value.
分类号：
- 相关文献
作者其他论文 更多>>
-
Structural characterization and hypolipidemic activity of a hetero-galactan purified from Sanghuangporus vaninii based on modulation of TLR4/NF-κB pathway
作者：Hao, Jie;Zhu, Yanfeng;Li, Zhige;Wang, Lu;Qu, Yidi;Wang, Di;Zhang, Yongfeng;Li, Lanzhou;Wang, Di;Yu, Hailong;Qi, Liangliang
关键词：Sanghuangporus vaninii; Polysaccharide; Hypolipidemic efficacy; Inflammation; TLR4/NF-kappa B
-
Baicalein inhibits PRRSV through direct binding, targeting EGFR, and enhancing immune response
作者：Wu, Jing;Lu, Qi;Hou, Jing;Qiu, Yueqin;Tian, Min;Wang, Li;Gao, Kaiguo;Yang, Xuefen;Jiang, Zongyong;Wu, Jing;Lu, Qi;Hou, Jing;Qiu, Yueqin;Tian, Min;Wang, Li;Gao, Kaiguo;Yang, Xuefen;Jiang, Zongyong;Wu, Jing;Lu, Qi;Hou, Jing;Qiu, Yueqin;Tian, Min;Wang, Li;Gao, Kaiguo;Yang, Xuefen;Jiang, Zongyong;Wu, Jing;Lu, Qi;Hou, Jing;Qiu, Yueqin;Tian, Min;Wang, Li;Gao, Kaiguo;Yang, Xuefen;Jiang, Zongyong
关键词：Piglets; PRRSV; baicalein; EGFR; MD simulation
-
Effect of Gardeniae Fructus Powder on Growth Performance, Antioxidant Capacity, Intestinal Barrier Function, and Colonic Microbiota of Weaned Piglets
作者：Liu, Shilong;Ma, Ming;Guo, Shining;Liu, Shilong;Tian, Min;Qiu, Yueqin;Tang, Jiaxi;Hou, Jing;Lu, Qi;Tian, Chaoyang;Ye, Guohao;Wang, Li;Gao, Kaiguo;Jiang, Zongyong;Yang, Xuefen;Liu, Shilong;Tian, Min;Qiu, Yueqin;Tang, Jiaxi;Hou, Jing;Lu, Qi;Tian, Chaoyang;Ye, Guohao;Wang, Li;Gao, Kaiguo;Jiang, Zongyong;Yang, Xuefen;Liu, Shilong;Tian, Min;Qiu, Yueqin;Tang, Jiaxi;Hou, Jing;Lu, Qi;Tian, Chaoyang;Ye, Guohao;Wang, Li;Gao, Kaiguo;Jiang, Zongyong;Yang, Xuefen;Liu, Shilong;Tian, Min;Qiu, Yueqin;Tang, Jiaxi;Hou, Jing;Lu, Qi;Tian, Chaoyang;Ye, Guohao;Wang, Li;Gao, Kaiguo;Jiang, Zongyong;Yang, Xuefen
关键词：piglet; Gardeniae Fructus; antioxidant capacity; intestinal barrier function; colonic microbiota
-
A cross-scale transfer learning framework: prediction of SOD activity from leaf microstructure to macroscopic hyperspectral imaging
作者：Hao, Jie;Zhang, Yiyang;Cao, Yune;Wu, Longguo;Hao, Jie;Yan, Yan;Zhang, Yao;Zhang, Yao;Wu, Longguo
关键词：superoxide dismutase; microhyperspectral imaging technology; heterogeneous two-dimensional correlation spectra; CNN-LSTM algorithm; transfer learning
-
Effects of Patulin Stress on the Physiology, Fermentation Performance, and Aroma Profile of Saccharomyces cerevisiae During Fermentation
作者：Yang, Chao;Huang, Lingxuan;Huang, Kuo;Peng, Bangzhu;Yang, Chao;Seah, Stephen Y. K.;Huang, Kuo;Peng, Bangzhu;Peng, Bangzhu
关键词：aroma components; fermentation characteristics; patulin; physiological;
Saccharomyces cerevisiae -
Fall Armyworm-Induced Secondary Metabolites in Sorghum Defend Against Its Attack
作者：Zhao, Juan-Ying;Wang, Huiyan;Zhang, Yizhong;Zhao, Juan-Ying;Wang, Huiyan;Zhang, Yizhong;Lu, Qi;Sun, Jiang;Sun, Li-Yuan;Ma, Ruiyan;Wang, Yuanxin;Hu, Jun;Jia, Dong;Yang, Jun;Ma, Ruiyan;Wang, Yuanxin;Hu, Jun;Jia, Dong;Yang, Jun
关键词：sorghum;
Spodoptera frugiperda ; secondary metabolites; defense mechanism; antifeedant -
Methyl 2-methylbutyrate produced by Streptomyces saccharicans strain ZBY1-20 inhibits Monilinia fructicola causing peach brown rot
作者：Chen, Shan;Chen, Meijun;Mu, Rong;Yang, Haorong;Liu, Mengfen;Ma, Guanhua;Sun, Xianchao;Dong, Guoju;Chen, Guokang;Chen, Meijun;Zheng, Yang;Jiang, Jihong;Huang, Kuo;Huang, Kuo;Ren, Jiequn
关键词：Peach brown rot; Monilinia fructicola; Streptomyces diastaticus ZBY1-20; Methyl 2-methylbutyrate
