Xylose-Driven Metabolic Reprogramming in Saccharomyces cerevisiae for Enhancing p-Coumaric Acid Production

文献类型： 外文期刊

第一作者： Zhao, Yifei

作者： Zhao, Yifei;Xiao, Zhiqiang;Wang, Yongtong;Tan, Xinjia;Zhang, Siqi;Lu, Qiyuan;Hu, Fanglin;Zuo, Shasha;Shan, Yang;Li, Gaoyang;Zhao, Yifei;Xiao, Zhiqiang;Wang, Yongtong;Tan, Xinjia;Zhang, Siqi;Lu, Qiyuan;Hu, Fanglin;Zuo, Shasha;Shan, Yang;Liu, Juan;Li, Gaoyang;Zhao, Yifei;Xiao, Zhiqiang;Wang, Yongtong;Tan, Xinjia;Zhang, Siqi;Lu, Qiyuan;Hu, Fanglin;Zuo, Shasha;Shan, Yang;Liu, Juan;Li, Gaoyang

作者机构：

关键词： xylose; Saccharomyces cerevisiae; xylotrophic yeast; ethanol overflow metabolism; Crabtree effect; p-coumaric acid

期刊名称：ACS SYNTHETIC BIOLOGY （ 影响因子：3.9； 五年影响因子：4.3 ）

ISSN： 2161-5063

年卷期： 2025 年 14 卷 7 期

页码：

收录情况： SCI

摘要： Xylose, the second most abundant sugar in nature, has garnered increasing attention as a promising carbon source for microbial fermentation in recent years. However, the unpredictable and inefficient metabolism of xylose in Saccharomyces cerevisiae has limited its practical application. In this study, we developed a xylotrophic strain through strategic integration of the xylose isomerase pathway, increasing xylose isomerase activity and identifying optimal transporters. Characterization of the modified strain demonstrated an 11.84-fold increase in ATP content under xylose conditions compared to glucose. This was achieved by redirecting carbon flux away from glycolysis, which resulted in a reduced level of ethanol and glycerol production. To demonstrate the industrial relevance of this platform, we applied the optimized strain to synthesize p-coumaric acid (p-CA). After process refinement, the strain achieved a final titer of 1293.15 mg/L p-CA using xylose as the sole carbon source, representing a 68.29% yield improvement compared to the glucose mode. To the best of our knowledge, this represents the highest reported to date for p-CA production from xylose alone. This study highlights the metabolic advantages of xylotrophic yeast and demonstrates the potential of leveraging these advantages for efficient p-CA synthesis, paving the way for the sustainable valorization of xylose into high-value natural products.

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