Three novel immunogenic proteins determined through 2-Dimensional electrophoresis and mass spectrometry with immune serum confer protection against challenge with porcine Pasteurella multocida in mouse models

文献类型： 外文期刊

第一作者： Wang, Fei

作者： Wang, Fei;Wang, Xueying;Ai, Weicheng;Zeng, Dongzhu;Liang, Wan;Hua, Lin;Liu, Heng;Wang, Xiangru;Chen, Huanchun;He, Qigai;Peng, Zhong;Wu, Bin;Wang, Fei;Wang, Xueying;Ai, Weicheng;Liang, Wan;Hua, Lin;Wang, Xiangru;Chen, Huanchun;He, Qigai;Peng, Zhong;Wu, Bin;Liang, Wan;Tian, Yongxiang

作者机构：

关键词： Pasteurella multocida; Novel immunogenic proteins; Immunogenicity; Protective efficacy; 2-DE MALDI-TOF MS

期刊名称：RESEARCH IN VETERINARY SCIENCE （ 影响因子：2.534； 五年影响因子：2.382 ）

ISSN： 0034-5288

年卷期： 2021 年 136 卷

页码：

收录情况： SCI

摘要： Pasteurella multocida is an important zoonotic pathogen that causes multiple diseases in both animals and humans. Test of good immunogenic proteins is beneficial for vaccine development and disease control. In the present study, we determined four novel immunogenic proteins of P. multocida by using 2-DE MALDI-TOF MS with immune serum. These four proteins included a trimethylamine-N-oxide reductase TorA, a translation elongation factor Ts, a phosphoglyceromutase PGAM, and a peroxiredoxin PrX. Among these proteins, TorA, Prx, and PGAM were successfully expressed by using E. coli. Western-blotting assays showed that recombinant TorA, Prx, and/or PGAM displayed good reactions with infectious sera of P. multocida serogroups A, B, D and F. Immunization of either rTorA, rPrx, and/or rPGAM induced significantly high levels of antibodies as well as IFN-gamma, IL-4 and IL-10 in mice (P < 0.01). Protective efficacy tests revealed that vaccination of either rTorA, rPrx, and/or rPGAM protected 60% similar to 80% of the tested mice against the challenge with P. multocida field isolate. Our results obtained from the present study suggest that these three proteins could be tested as good vaccine candidates against P. multocida infections.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序