Functional Equivalence of Insulin and IGF-1 in the In Vitro Culture of Chicken Primordial Germ Cells

文献类型： 外文期刊

第一作者： Liu, Xin

作者： Liu, Xin;Wu, Jun;Peng, Yixiu;Liu, Guangzheng;Jin, Kai;Niu, Yingjie;Chen, Guohong;Li, Bichun;Zuo, Qisheng;Liu, Xin;Wu, Jun;Peng, Yixiu;Liu, Guangzheng;Jin, Kai;Niu, Yingjie;Chen, Guohong;Li, Bichun;Zuo, Qisheng;Song, Jiuzhou;Han, Wei;Li, Bichun

作者机构：

关键词： chicken PGCs; IGF-1; migration; establishment efficiency

期刊名称：GENES （ 影响因子：2.8； 五年影响因子：3.2 ）

ISSN：

年卷期： 2025 年 16 卷 5 期

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收录情况： SCI

摘要： Background: Chicken Primordial Germ Cells (PGCs) are one of the few germ cells that can be cultured for a long time in vitro, but challenges remain such as low culture efficiency and unclear roles of nutrient factors and signaling pathways. Method: In this study, protein kinase B (AKT) pathway activator insulin-like growth factor 1 (IGF-1) was screened for its ability to promote cell proliferation by transcriptome results using various inhibitors of pathway activation. The effects of IGF-1 on PGCs were evaluated through EdU assays, qRT-PCR, flow cytometry, and migration experiments. Results: This study systematically examined the effects of insulin and IGF-1 on the proliferation, cell cycle, ferroptosis, migration capacity, and establishment efficiency of PGCs. The findings demonstrated that IGF-1 exhibited comparable effects to insulin and could effectively replace insulin in PGC culture systems. Conclusions: The research results are expected to provide a solid theoretical basis for optimizing the chicken PGC cultivation system and promoting its practical application.

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