Identification and possible mechanisms of Kitasatospora purpeofusca conferring biocontrol potential against Rhizoctonia solani

文献类型： 外文期刊

第一作者： Zhong, Jie

作者： Zhong, Jie;Sui, Wen Wen;Li, Xiao Gang;Zhu, Jun Zi;Zhang, Zhuo

作者机构：

关键词： Nicotiana tabacum; Rhizoctonia solani; Biological control; Whole genome sequencing; Antifungal mechanism

期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：4.0； 五年影响因子：4.5 ）

ISSN： 0048-3575

年卷期： 2025 年 213 卷

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收录情况： SCI

摘要： Rhizoctonia solani is a widespread and destructive plant pathogenic fungus that infects numerous important crops, including tobacco, where it causes tobacco target spot, a disease that severely impacts production. In this study, a strain ZZ-48, isolated from the rhizosphere soil of forests, exhibited strong antifungal activity with a 79.39 % inhibition rate against R. solani and various other fungal pathogens. Strain ZZ-48 was identified as Kitasatospora purpureofusca based on its phenotypic, physiological and biochemical, and genetic characteristics. This strain significantly reduced the development of tobacco target spot both in vitro and in vivo. ZZ-48 treatment significantly disrupted the mycelia structure of R. solani. Furthermore, the culture filtrate disrupted plasma membrane integrity and promoted reactive oxygen species accumulation and lipid peroxidation in the mycelia of R. solani. Additionally, ZZ-48 secreted multiple extracellular enzymes and siderophores. Bioactive metabolites were inferred to be present in the crude lipopeptide extracts, which exhibited a median effect concentration (EC50) value of 1.60 +/- 0.18 mu g/mL. Furthermore, several key biosynthetic gene clusters (BCGs) associated with the synthesis of bioactive metabolites were found in the genome of ZZ-48 using antiSMASH. Overall, strain ZZ-48 holds significant potential as a biocontrol agent for managing tobacco target spot.

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