Novel Calcium-Binding Peptide from Bovine Bone Collagen Hydrolysates and Its Potential Pro-Osteogenic Activity via Calcium-Sensing Receptor (CaSR)

文献类型： 外文期刊

第一作者： Qi, Liwei

作者： Qi, Liwei;Zhang, Hongru;Guo, Yujie;Zhang, Chunhui;Zhang, Hongru;Xu, Yang

作者机构：

关键词： calcium-binding capacity; calcium-binding peptide; calcium-sensing receptor; collagen hydrolysates; osteogenic activity

期刊名称：MOLECULAR NUTRITION & FOOD RESEARCH （ 影响因子：5.2； 五年影响因子：6.2 ）

ISSN： 1613-4125

年卷期： 2024 年 68 卷 4 期

页码：

收录情况： SCI

摘要： ScopeThis paper aims to explore the osteogenic activity and potential mechanism of the peptide-calcium chelate, and provides a theoretical basis for peptide-calcium chelates as functional foods to prevent or improve osteoporosis.Methods and resultsIn this research, a novel peptide (Phe-Gly-Leu, FGL) with a high calcium-binding capacity is screened from bovine bone collagen hydrolysates (CPs), calcium binding sites of which mainly included carbonyl, amino and carboxyl groups. The FGL-Ca significantly enhances the osteogenic activity of MC3T3-E1 cells (survival rate, differentiation, and mineralization). The results of calcium fluorescence labeling and molecular docking show that FGL-Ca may activate calcium-sensing receptor (CaSR), leading to an increase in intracellular calcium concentration, then enhancing osteogenic activity of MC3T3-E1 cells. Further research found that FGL-Ca significantly promotes the mRNA and protein expression levels of CaSR, transforming growth factor beta (TGF-beta 1), TGF-beta-type II receptor (T beta RII), Smad2, Smad3, osteocalcin (OCN), alkaline phosphatase (ALP), osteoprotegrin (OPG), and collagen type I (COLI). Subsequently, in the signal pathway intervention experiment, the expression levels of genes and proteins related to the TGF-beta 1/Smad2/3 signaling pathway that are promoted by FGL-Ca are found to decrease.ConclusionsThese results suggest that FGL-Ca may activate CaSR, increase intracellular calcium concentration, and activate TGF-beta 1/Smad2/3 signaling pathway, which may be one of the potential mechanisms for enhancing osteogenic activity. A novel calcium-binding peptide (FGL) with a high calcium-binding capacity is screened from bovine bone collagen hydrolysates. FGL-Ca can significantly enhance the osteogenic activity of MC3T3-E1 cells. The potential mechanism is that FGL-Ca can activate CaSR to increase intracellular calcium ions and induce TGF-beta 1 secretion, which activates the TGF-beta 1/Smad2/3 pathway and enhances osteogenic activity of MC3T3-E1 cells.image

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