Ultrasensitive Ribo-seq reveals translational landscapes during mammalian oocyte-to-embryo transition and pre-implantation development

文献类型： 外文期刊

第一作者： Xiong, Zhuqing

作者： Xiong, Zhuqing;Xu, Kai;Kong, Feng;Wang, Qiujun;Zou, Zhuoning;Liu, Ling;Ji, Shuyan;Zhang, Hongmei;Yu, Guang;Liu, Bofeng;Wang, Lijuan;Xie, Wei;Xiong, Zhuqing;Li, Fajin;Xu, Kai;Kong, Feng;Wang, Qiujun;Zou, Zhuoning;Liu, Ling;Ji, Shuyan;Zhang, Hongmei;Fang, Jianhuo;Yu, Guang;Liu, Bofeng;Wang, Lijuan;Xie, Wei;Lin, Zili;Quan, Yujun;Li, Lei;Quan, Yujun;Li, Lei;Sha, Qian-qian;Li, Fajin;Fang, Jianhuo;Yang, Xuerui;Li, Fajin;Fang, Jianhuo;Yang, Xuerui;Chen, Yuling;Deng, Haiteng;Wang, Huili;Fan, Heng-yu;Fan, Heng-yu;Fan, Heng-yu

作者机构：

期刊名称：NATURE CELL BIOLOGY （ 影响因子：28.213； 五年影响因子：28.123 ）

ISSN： 1465-7392

年卷期： 2022 年 24 卷 6 期

页码：

收录情况： SCI

摘要： In mammals, translational control plays critical roles during oocyte-to-embryo transition (OET) when transcription ceases. However, the underlying regulatory mechanisms remain challenging to study. Here, using low-input Ribo-seq (Ribo-lite), we investigated translational landscapes during OET using 30-150 mouse oocytes or embryos per stage. Ribo-lite can also accommodate single oocytes. Combining PAIso-seq to interrogate poly(A) tail lengths, we found a global switch of translatome that closely parallels changes of poly(A) tails upon meiotic resumption. Translation activation correlates with polyadenylation and is supported by polyadenylation signal proximal cytoplasmic polyadenylation elements (papCPEs) in 3 ' untranslated regions. By contrast, translation repression parallels global de-adenylation. The latter includes transcripts containing no CPEs or non-papCPEs, which encode many transcription regulators that are preferentially re-activated before zygotic genome activation. CCR4-NOT, the major de-adenylation complex, and its key adaptor protein BTG4 regulate translation downregulation often independent of RNA decay. BTG4 is not essential for global de-adenylation but is required for selective gene de-adenylation and production of very short-tailed transcripts. In sum, our data reveal intimate interplays among translation, RNA stability and poly(A) tail length regulation underlying mammalian OET. Using an optimized Ribo-seq protocol that is applicable for low-input samples, Xie, Li and colleagues revealed the translation landscape during oocyte-to-embryo transition and in pre-implantation embryos.

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