A high-affinity nitrate transporter PaNRT2.1 mediates dark septate endophyte (DSE) dependent nitrogen assimilation in sweet cherry roots

文献类型： 外文期刊

第一作者： Wu, Fanlin

作者： Wu, Fanlin;Qu, Dehui;Zhao, Xiaohui;Su, Hongyan;Zhu, Dongzi;Zhang, Xu;Wang, Lei

作者机构：

关键词： DSE; PaNRT2; 1; Sweet cherry; Symbiosis; PaCRK10

期刊名称：PLANT AND SOIL （ 影响因子：4.9； 五年影响因子：5.2 ）

ISSN： 0032-079X

年卷期： 2023 年

页码：

收录情况： SCI

摘要： Background and aimsLow nitrogen (N) soil environment is one of the main factors limiting crop yield and horticultural plant growth. Dark septate endophytes (DSEs) are co-occurring in 80% of terrestrial plants and they can promote the absorption of N. However, few data on the contribution of DSEs symbiosis to plant N acquisition and their molecular mechanisms are available.MethodsThe growth, N contents and expression of N related genes of Gisela 5, a sweet cherry rootstock colonized with and without DSE fungus S16, were measured under low and normal nitrate conditions. Subsequently, molecular identification and functional study of PaNRT2.1, a nitrate transporter highly responsive to S16, were conducted.ResultsIn this study, we found that S16 promoted the growth and N uptake of Gisela 5 seedlings under low and normal nitrate conditions, and induced the expression of the nitrate transporter gene PaNRT2.1 in roots. The expression of PaNRT2.1 significantly affected the nitrate uptake of Gisela 5 roots and the symbiosis with S16 under low nitrate conditions. Transcriptome analysis showed that a receptor-like kinase gene PaCRK10 was significantly induced during S16 symbiosis and PaNRT2.1 overexpression, indicating a signal transduction between DSE colonization with nitrate uptake. Further studies showed that there was a strong interaction between PaNRT2.1 and PaCRK10.ConclusionsTaken together, our results provide a deeper understanding of how DSE promoted N uptake by sweet cherry rootstock Gisela 5 and a more promising approach for improving N uptake efficiency through plant-microbe interactions.

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