Transcriptome profiling of differentiating adipose-derived stem cells across species reveals new genes regulating adipogenesis

文献类型： 外文期刊

第一作者： Han, Jiangang

作者： Han, Jiangang;Li, Xiaojie;Liang, Benmeng;Ma, Sijia;Pu, Yabin;Ma, Yuehui;Jiang, Lin;Han, Jiangang;Machugh, David E.;Li, Xiaojie;Liang, Benmeng;Pu, Yabin;Ma, Yuehui;Jiang, Lin;Ma, Sijia;Yu, Fuqing;Lu, Jian;Machugh, David E.

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关键词： Adipose-derived stem cell; Multilineage differentiation; Adipogenesis; Transcription factor; Transcriptomics

期刊名称：BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS （ 影响因子：4.8； 五年影响因子：5.0 ）

ISSN： 1388-1981

年卷期： 2023 年 1868 卷 10 期

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收录情况： SCI

摘要： Adipose-derived stem cells (ADSCs) that are enriched in adipose tissue with multilineage differentiation potential have become an important tool in therapeutic research and tissue engineering. Certain breeds of sheep exhibit a unique fat tail trait such that tail tissue accounts for approximately 10 % of body weight and can provide an excellent source of ADSCs. Here, we describe isolation of primary ADSCs from ovine embryonic fat tail tissues that displayed high self-renewal capacity, multilineage differentiation and excellent adipogenic ability. Through transcriptome analysis covering ADSCs differentiating into adipocytes, 37 transcription factors were involved in early transcriptional events that initiate a regulatory cascade of adipogenesis; the entire adipogenic activity consists of a reduction in proliferation ability and upregulation of genes related to lipid generation and energy metabolism, as well as several genes associated with myogenesis. Furthermore, Comparative transcriptome analysis across species (sheep, human, and mouse) revealed enhanced basal metabolic ability in differentiating ovine ADSCs, which may relate to the excellent adipogenic capability of these cells. We also identified a small evolutionarily conserved gene set, consisting of 21 and 22 genes exhibiting increased and decreased expression, respectively. Almost half (20) of these genes have not previously been reported to regulate adipogenesis in mammals. In this study, we identified important regulators that trigger ovine adipocyte differentiation, main biological pathways involved in adipogenesis as well as the evolutionarily conserved genes governing adipogenic process across species. Our study provides a novel excellent biomaterial and novel genes regulating adipogenesis for cellular transplantation therapy and investigations of fat metabolism.

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