Revelation of benzo(a)pyrene-induced latent toxicity mechanism to intestinal histomorphology and oxidative status for common carp (Cyprinus carpio) through transcriptional and single-cell sequencing

文献类型： 外文期刊

第一作者： Hao, Qirui

作者： Hao, Qirui;Chen, Zhongxiang;Fan, Ze;Wu, Song;Bai, Shuyan;Du, Ningning;Li, Chenhui;Wang, Peng;Huang, Li;Gao, Lei;Hao, Qirui;Chen, Zhongxiang;Wu, Song;Bai, Shuyan;Du, Ningning;Li, Chenhui;Wang, Peng;Huang, Li;Gao, Lei;Hao, Qirui;Chen, Zhongxiang;Wu, Song;Bai, Shuyan;Du, Ningning;Li, Chenhui;Wang, Peng;Huang, Li;Gao, Lei;Hao, Qirui;Chen, Zhongxiang;Wu, Song;Bai, Shuyan;Du, Ningning;Li, Chenhui;Wang, Peng;Huang, Li;Gao, Lei;Fan, Ze;Zhao, Rongwei

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关键词： Benzo( a )pyrene exposure; Common carp; GSH signaling pathway; ADP signaling pathway; Lysosome-phagosome pathway; Myeloid cells

期刊名称：AQUACULTURE （ 影响因子：4.5； 五年影响因子：4.6 ）

ISSN： 0044-8486

年卷期： 2024 年 585 卷

页码：

收录情况： SCI

摘要： Common carp (Cyprinus carpio) (mean weight 50.0 +/- 10.0 g) were exposed for 15 d with benzo[a]pyrene (B[a]P) concentrations of 0, 2.5 and 25 mu g/kg in the water. For markers of oxidative stress, intestinal contents of glutathione (GSH) and malondialdehyde and activities of catalase and superoxide dismutase were significantly elevated after 15-d B[a]P exposure. For intestinal histology and ultrastructure analysis, intestinal histiocytes not only swelled, but also intensified blood aggregation, resulting in hematoma after exposed to high B[a]P concentration. For intestinal transcriptome response, GSH signaling pathway was abnormally activated after exposure to B[a]P, thereby resulting in intestinal oxidative damage. Meanwhile, exposure to B[a]P suppressed intestinal platelet activation depending on ADP signaling pathway. Exposure to high B[a]P concentration induced intestinal cellular edema through the suppression of Lysosome-Phagosome pathway. For scRNA-seq results, myeloid cells under B[a]P stress, despite having evolved into cell types with distinct specialties, still retained innate immune functions such as Macrophage_3. The present study provides valuable and front-edged insights for developing sustainable solutions to address the challenges posed by PAHs waste and B[a]P contamination on aquaculture system.

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