Improvement of plant growth and fruit quality by introducing a phosphoribosylpyrophosphate synthetase mutation into Methylorubrum populi

文献类型： 外文期刊

第一作者： Zhang, Cong

作者： Zhang, Cong;Yao, Lu;Zhang, Ming-Ming;Tian, Dan-Dan;Wu, Jing;Hu, Yu-Zheng;Ma, Zeng-Xin;Tan, Ling-Ling;Yang, Song;Zhang, Cong;Ma, Zeng-Xin;Tan, Ling-Ling;Yang, Song;Zhang, Cong;Ma, Zeng-Xin;Tan, Ling-Ling;Yang, Song;Yao, Lu;Bao, Kai;Yang, Song;Yang, Song

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关键词： phyllosphere colonization; Methylorubrum populi; phosphoribosylpyrophosphate synthetase; cucumber; quality improvement

期刊名称：JOURNAL OF APPLIED MICROBIOLOGY （ 影响因子：3.2； 五年影响因子：3.8 ）

ISSN： 1364-5072

年卷期： 2025 年 136 卷 1 期

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收录情况： SCI

摘要： Aims The aim of this study was to evaluate the impact of the introduction of a phosphoribosylpyrophosphate synthetase (PRS) mutation into a plant growth-promoting strain of Methylorubrum on the enhancement of phyllosphere colonization, with the ultimate goal of improving plant growth and quality.Methods and results A strain of Methylorubrum populi (named HS04) was isolated from the groundnut leaves and found to process the plant-promoting traits, including the ability to produce indole acetic acid, siderophore, 1-aminocyclopropane-1-carboxylate deaminase, and to fix nitrogen. The application via foliar spray significantly increased the fresh weight of cucumber seedlings cultivated in a standard growth chamber, with 43.0% higher than the control group. Genomic analysis revealed that the presence of an array of genes involved in plant growth promotion, including accD, aldB, and ltaE, as well as potential nitrogen-fixation-related genes, including nifA, bchlLNB, and bchXYZ, in the HS04 strain. The introduction of the PRS mutation (an aspartic acid to an asparagine residue, D38N) in the HS04 strain (named HS04PTR) enhanced the utilization capacity of low concentrations of methanol and multi-carbon sources (C2-C5 carbon sources). The HS04PTR strain indicated a notable enhancement in the phyllosphere colonization, with the subsequent application further promoting the growth of cucumber seedlings. An agricultural solar greenhouse experiment was thus performed to assess the efficiency of the HS04PTR strain, sprayed at low abundance, in improving the growth and quality of cucumber plants, including vitamin C, reducing sugars, and total sugars.Conclusions Our findings provide insights into the potential of Methylorubrum/Methylobacterium strains with the PRS mutations as an efficient inoculant for advantageous agricultural applications.

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