Engineering, expression, and immuno-characterization of recombinant protein comprising multi-neutralization sites of rabies virus glycoprotein

文献类型： 外文期刊

第一作者： Li, Xiangdong

作者： Li, Xiangdong;Luo, Jinyan;Shen, Yang;Qiu, Yafeng;Wang, Xiaodu;Deng, Xufang;Ding, Chan;Ma, Zhiyong;Wang, Shuiming;Liu, Xuehui;Bao, Weidong;Liu, Peihong;Zhou, Jinping

作者机构：

关键词： G protein;Neutralization sites;Prokaryotic expression;Rabies virus

期刊名称：PROTEIN EXPRESSION AND PURIFICATION （ 影响因子：1.65； 五年影响因子：1.548 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The rabies virus (RV) glycoprotein (G protein) induces neutralizing antibodies, which are important in protection against rabies. In the present study, three gene fragments that encode polypeptides (corresponding to amino acid residues 19-60, 181-219, and 300-458) comprising the linear neutralization sites of the G protein were spliced together in tandem by PCR-based site-directed mutagenesis and heterologously expressed in Escherichia coli (DE3). The recombinant protein (designated rRVg) was purified under denaturing conditions and solubilized by stepwise dialysis against an alkaline buffer (0.05 M Na_2CO_3 pH 9.6). Western blot analysis of the antigenicity of rRVg showed that it was recognized by rabies-immune serum. Competitive neutralization assay revealed that rRVg significantly reduced the RV-neutralizing activity of the rabies-immune serum. These results show potential of rRVg as a diagnostic antigen for detecting RV-neutralizing antibodies in immunized hosts.

分类号： Q51

  • 相关文献

[1]An inactivated recombinant rabies CVS-11 virus expressing two copies of the glycoprotein elicits a higher level of neutralizing antibodies and provides better protection in mice. Xue, Xiang-Hong,Li, Ling,Yang, Song-Tao,Xia, Xian-Zhu,Xue, Xiang-Hong,Zheng, Xue-Xing,Wang, Hua-Lei,Ma, Jin-Zhu,Li, Ling,Gai, Wei-Wei,Wang, Tie-Cheng,Yang, Song-Tao,Xia, Xian-Zhu,Zheng, Xue-Xing,Ma, Jin-Zhu.

[2]Efficacy of DNA Immunization with F and G Protein Genes of Nipah Virus. Wang, Xijun,Ge, Jinying,Hu, Sen,Wang, Qinghua,Wen, Zhiyun,Chen, Hualan,Bu, Zhigao.

[3]A dwarfing mutant caused by deactivation function of alpha subunit of the heterotrimeric G-protein in rice. Yang, De-wei,Zheng, Xiang-hua,Cheng, Chao-ping,Lu, Li-bin,Liu, Chen-de,Ye, Ning,Zeng, Mei-juan,Ye, Xin-fu,Wang, Wen-da,Xing, Deng-hui.

[4]Chimeric rabies glycoprotein with a transmembrane domain and cytoplasmic tail from Newcastle disease virus fusion protein incorporates into the Newcastle disease virion at reduced levels. Yu, Gui Mei,Zu, Shu Long,Zhou, Wei Wei,Wang, Xi Jun,Shuai, Lei,Wang, Xue Lian,Ge, Jin Ying,Bu, Zhi Gao. 2017

[5]Seroprevalence of Rabies Virus Antibodies in Bats from Southern China. Jiang, Yu,Wang, Lili,Han, Xiaohu,Xia, Xianzhu,Tu, Changchun,Wang, Lili,Zhao, Fuguang,Lu, Zongji,Xuan, Hua. 2010

[6]Recombinant rabies virus expressing the H protein of canine distemper virus protects dogs from the lethal distemper challenge. Wang, Feng-Xue,Zhang, Shu-Qin,Zhu, Hong-Wei,Yang, Yong,Sun, Na,Tan, Bin,Li, Zhen-Guang,Cheng, Shi-Peng,Wen, Yong-Jun,Li, Zhen-Guang,Fu, Zhen F..

[7]Rabies virus nucleoprotein expressed in silkworm pupae at high-levels and evaluation of immune responses in mice. Yin, Xiangping,Li, Zhiyong,Li, Jiangtao,Zhang, Yun,Li, Xuerui,Li, Baoyu,Yang, Bin,Lan, Xi,Jiao, Wenqiang,Liu, Jixing,Yi, Yongzhu,Li, Yinv,Zhang, Zhifang.

[8]Genetically modified rabies virus ERA strain is safe and induces long-lasting protective immune response in dogs after oral vaccination. Shuai, Lei,Wang, Xijun,Ge, Jinying,Wen, Zhiyuan,Chen, Weiye,Qin, Lide,Bu, Zhigao,Feng, Na,Xia, Xianzhu.

[9]Generation of a recombinant rabies Flury LEP virus carrying an additional G gene creates an improved seed virus for inactivated vaccine production. Tao, Lihong,Ge, Jinying,Wang, Xijun,Wen, Zhiyuan,Zhai, Hongyue,Hua, Tao,Zhao, Bolin,Kong, Dongni,Bu, Zhigao,Yang, Chinglai. 2011

[10]Immunogenicity of multi-epitope-based vaccine candidates administered with the adjuvant Gp96 against rabies. Niu, Yange,Yang, Limin,Qu, Hongren,Li, Jing,Liu, Wenjun,Niu, Yange,Liu, Wenjun,Liu, Ye,Hu, Rongliang,Liu, Ye,Hu, Rongliang,Zhao, Jingyi,Li, Jing. 2016

[11]Immunoenhancement with flagellin as an adjuvant to whole-killed rabies vaccine in mice. Xiao, Xing-xing,Zhang, Yun,Liu, Ji-xing,Wei, Qiao-lin,Yin, Xiang-ping.

[12]Enhancement of blood-brain barrier permeability is required for intravenously administered virus neutralizing antibodies to clear an established rabies virus infection from the brain and prevent the development of rabies in mice. Huang, Chien-Tsun,Li, Zhenguang,Huang, Ying,Zhang, Guoqing,Zhou, Ming,Chai, Qingqing,Fu, Zhen F.,Li, Zhenguang,Wu, Hua,Fu, Zhen F..

[13]Inactivated Recombinant Rabies Viruses Displaying Canine Distemper Virus Glycoproteins Induce Protective Immunity against Both Pathogens. Budaszewski, Renata da Fontoura,Budaszewski, Renata da Fontoura,Sawatsky, Bevan,Kraemer, Beate,von Messling, Veronika,Sawatsky, Bevan,von Messling, Veronika,Hudacek, Andrew,Yin, Xiangping,Schnell, Matthias J.,Yin, Xiangping.

[14]Characterization of a virulent dog-originated rabies virus affecting more than twenty fallow deer (Dama dama) in Inner Mongolia, China. Zhu, Hongwei,Shao, Xiqun,Ba, Hengxing,Wang, Fengxue,Yang, Yong,Sun, Na,Ren, Jingqiang,Cheng, Shipeng,Wen, Yongjun,Chen, Xiaoyun,Wang, Hualei.

[15]Genetically modified rabies virus-vectored Ebola virus disease vaccines are safe and induce efficacious immune responses in mice and dogs. Shuai, Lei,Wang, Xijun,Wen, Zhiyuan,Ge, Jinying,Wang, Jinliang,Zhao, Dandan,Bu, Zhigao.

[16]Generation of Recombinant Rabies Virus CVS-11 Expressing eGFP Applied to the Rapid Virus Neutralization Test. Xue, Xianghong,Zheng, Xuexing,Liang, Hongru,Feng, Na,Zhao, Yongkun,Gao, Yuwei,Wang, Hualei,Yang, Songtao,Xia, Xianzhu,Xue, Xianghong,Yang, Songtao,Xia, Xianzhu,Zheng, Xuexing,Xia, Xianzhu. 2014

[17]Aptamers targeting rabies virus-infected cells inhibit street rabies virus in vivo. Liang, Hong-Ru,Liang, Hong-Ru,Hu, Gu-Qiu,Li, Ling,Gao, Yu-Wei,Yang, Song-Tao,Xia, Xian-Zhu. 2014

[18]Molecular characterization of China rabies virus vaccine strain. Jiao, Wenqiang,Yin, Xiangping,Li, Zhiyong,Lan, Xi,Li, Xuerui,Tian, Xiaoting,Li, Baoyu,Yang, Bin,Zhang, Yun,Liu, Jixing. 2011

[19]Immunization with recombinant rabies virus expressing Interleukin-18 exhibits enhanced immunogenicity and protection in mice. Gai, Weiwei,Zheng, Wenwen,Song, Yanyan,Gai, Weiwei,Zheng, Xuexing,Wang, Chong,Wong, Gary. 2017

[20]Selection of an aptamer against rabies virus: A new class of molecules with antiviral activity. Liang, Hong-Ru,Liang, Hong-Ru,Hu, Gui-Qiu,Xue, Xiang-Hong,Li, Lu,Zheng, Xue-Xing,Gao, Yu-Wei,Yang, Song-Tao,Xia, Xian-Zhu.

作者其他论文 更多>>

微信小程序