Construction of a Bacillus thuringiensis engineered strain with high toxicity and broad pesticidal spectrum against coleopteran insects

文献类型： 外文期刊

第一作者： Liu, Jingjing

作者： Liu, Jingjing;Yan, Guixin;Shu, Changlong;Zhao, Can;Song, Fuping;Zhou, Lin;Ma, Junlan;Zhang, Jie;Liu, Chunqin;Huang, Dafang

作者机构：

关键词： Anomala corpulenta;Bacillus thuringiensis;Engineered strain;Holotrichia parallela;Homologous recombination

期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：4.813； 五年影响因子：4.697 ）

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收录情况： SCI

摘要： A newly engineered strain, denominated BIOT185, was constructed through integrating the cry8Ca2 gene into the endogenous plasmid of BT185 (contains cry8Ea1) by homologous recombination. The thermosensitive plasmid vector was eliminated by the rising temperature of recombinant cultures. No antibiotic gene or other unnecessary genes were introduced to the new strain. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blot analysis demonstrated that the cry8Ca2 gene was expressed normally and produced a 130-kDa protein in the BIOT185 strain. Bioassay results showed that the new strain had high toxicity to the pests Anomala corpulenta and Holotrichia parallela, which often damage the same fields.

分类号： Q939.9

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