Development of a loop-mediated isothermal amplification method for rapid detection of reticuloendotheliosis virus

文献类型： 外文期刊

第一作者： Deng, Xiaoyun

作者： Deng, Xiaoyun;Qi, Xiaole;Gao, Yulong;Wang, Yongqiang;Qin, Liting;Gao, Honglei;Gao, Li;Wang, Xiaomei;Deng, Xiaoyun

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关键词： viral isolation

期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）

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收录情况： SCI

摘要： A loop-mediated isothermal amplification (LAMP) method for rapid detection of reticuloendotheliosis virus (REV) was developed. The method used a set of two pairs of primers to amplify the pot gene for detecting REV, showing high specificity and sensitivity. The REV LAMP method did not cross-react with common avian DNA viruses (Marek's disease virus, chicken anaemia virus, avian leucosis virus of subgroup J). Additionally, the assay could detect different REV strains and had a detection limit of five copies and therefore a higher sensitivity than traditional PCR methods. Furthermore, the efficiency of LAMP for detection REV in clinical samples was comparable to PCR and viral isolation. The procedure of LAMP is simple and does not rely on any special equipment. The detection of REV by LAMP will be useful for detecting and controlling reticuloendotheliosis

分类号： R37

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